Rabbit anti-Human, Mouse PGK1 Polyclonal Antibody | anti-PGK1 antibody

PGK1, ID (PGK1, PGKA, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2) (AP)

Cat. Num. AAA6333035

• Gene Names: PGK1; PGKA; MIG10; HEL-S-68p
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PGK1; Polyclonal Antibody; ID (PGK1; PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2) (AP); Primer recognition protein 2; anti-PGK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PGK1 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the center region of human PGK1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hPGK1-G132 in Hela(lane 1), HepG2(lane 2) cell line lysates (35ug/lane). PGK1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PGK1 (arrow) using rabbit polyclonal PGK1 Antibody (G132). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PGK1 gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PGK1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PGK1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-PGK1 antibody

Antibodies; Protein Kinases

References

Lay, A. J., et al. Nature 408: 869-873 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 52788229
• NCBI GeneID: 5230
• UniProt Accession #: P00558; Q5J7W1; Q6IBT6; Q8NI87; A8K4W6; B7Z7A9
• Molecular Weight: 417
• NCBI Official Full Name: Phosphoglycerate kinase 1
• NCBI Official Synonym Full Names: phosphoglycerate kinase 1
• NCBI Official Symbol: PGK1
• NCBI Official Synonym Symbols: PGKA; MIG10; HEL-S-68p
• NCBI Protein Information: phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p
• UniProt Protein Name: Phosphoglycerate kinase 1
• Protein Family: Phosphoglycerate kinase
• UniProt Gene Name: PGK1
• UniProt Synonym Gene Names: PGKA; PRP 2
• UniProt Entry Name: PGK1_HUMAN

NCBI Description

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]

Uniprot Description

PGK1: an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein).

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.2.3

Chromosomal Location of Human Ortholog: Xq13.3

Cellular Component: membrane; cytosol

Molecular Function: protein binding; phosphoglycerate kinase activity; ATP binding

Biological Process: glycolysis; epithelial cell differentiation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; phosphorylation; gluconeogenesis

Disease: Phosphoglycerate Kinase 1 Deficiency