Rabbit PGAM1 Polyclonal Antibody | anti-PGAM1 antibody

PGAM1 Polyclonal Antibody

Cat. Num. AAA3013245

• Gene Names: PGAM1; PGAMA; PGAM-B; HEL-S-35
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: PGAM1; Polyclonal Antibody; PGAM1 Polyclonal Antibody; PGAM-B; PGAMA; BPG dependent PGAM 1; BPG dependent PGAM1; BPG-dependent PGAM 1; OTTHUMP00000059414; PGAM 1; PGAM A; PGAM B; PGAMB; Phosphoglycerate mutase 1; Phosphoglycerate mutase 1 brain; Phosphoglycerate mutase A; Phosphoglycerate mutase A nonmuscle form; Phosphoglycerate mutase isozyme B; anti-PGAM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PGAM1 polyclonal antibody detects endogenous levels of PGAM1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 254

• Applicable Applications for anti-PGAM1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; IHC: 1:50-1:100; FC/FACS: 1:50-1:100
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of PGAM1 on different lysates using anti-PGAM1 antibody at 1/2,000 dilution. Positive control:Lane 1: A431 Lane 2: A549Lane 3: Rat brain Lane 4: Mouse brain)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PGAM1 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-PGAM1 antibody

Members of the PGAM (phosphoglycerate mutase) family of proteins are important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism. They are responsible for catalyzing the transfer of phospho groups between the carbon atoms of phosphoglycerates. In mammals there are two types of PGAM isozymes: PGAM1 (also known as PGAMB) and PGAM2 (also known as PGAMA). In the cell, PGAM1 and PGAM2 exist as either homodimers or heterodimers and are responsible for the interconversion of 3-phosphoglycerate and 2-phosphoglycerate. PGAM2 homodimers are expressed in skeletal muscle, mature sperm cells and heart; PGAM1 homodimers are found in most other tissues; and PGAM1/PGAM2 heterodimers are found exclusively in the heart. PGAM4, also known as PGAM3, is a protein formerly considered to be specific to humans. Initially the PGAM4 gene was described as a pseudogene but it is now known to encode a functional protein at least 25 million years old. The gene encoding PGAM4 is believed to have originated by retrotransposition, with the original copy being the PGAM1 gene.

NCBI and Uniprot Product Information

• NCBI GI #: 130348
• NCBI GeneID: 5223
• UniProt Accession #: P18669; Q9DBJ1; P25113
• Molecular Weight: 29kDa
• NCBI Official Full Name: Phosphoglycerate mutase 1
• NCBI Official Synonym Full Names: phosphoglycerate mutase 1
• NCBI Official Symbol: PGAM1
• NCBI Official Synonym Symbols: PGAMA; PGAM-B; HEL-S-35
• NCBI Protein Information: phosphoglycerate mutase 1
• UniProt Protein Name: Phosphoglycerate mutase 1
• Protein Family: Phosphoglycerate mutase
• UniProt Gene Name: PGAM1
• UniProt Synonym Gene Names: PGAMA; PGAM-B
• UniProt Entry Name: PGAM1_HUMAN

NCBI Description

The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: EC 5.4.2.4; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; EC 3.1.3.13; Phosphatase (non-protein); EC 5.4.2.11

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; protein kinase binding

Biological Process: respiratory burst; glycolysis; dephosphorylation; regulation of glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; regulation of pentose-phosphate shunt; gluconeogenesis