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Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using PGAM1 antibody.)

Rabbit PGAM1 Polyclonal Antibody | anti-PGAM1 antibody

PGAM1 Polyclonal Antibody

Gene Names
PGAM1; PGAMA; PGAM-B; HEL-S-35
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PGAM1; Polyclonal Antibody; PGAM1 Polyclonal Antibody; PGAMA; PGAM-B; anti-PGAM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PGAM1 polyclonal antibody detects endogenous levels of PGAM1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
254
Applicable Applications for anti-PGAM1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant protein of human PGAM1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using PGAM1 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using PGAM1 antibody.)
Related Product Information for anti-PGAM1 antibody
Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 29kDa
NCBI Official Full Name
Phosphoglycerate mutase 1
NCBI Official Synonym Full Names
phosphoglycerate mutase 1
NCBI Official Symbol
PGAM1
NCBI Official Synonym Symbols
PGAMA; PGAM-B; HEL-S-35
NCBI Protein Information
phosphoglycerate mutase 1
UniProt Protein Name
Phosphoglycerate mutase 1
Protein Family
UniProt Gene Name
PGAM1
UniProt Synonym Gene Names
PGAMA; PGAM-B
UniProt Entry Name
PGAM1_HUMAN

NCBI Description

The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: EC 5.4.2.4; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; EC 3.1.3.13; Phosphatase (non-protein); EC 5.4.2.11

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; protein kinase binding

Biological Process: respiratory burst; glycolysis; dephosphorylation; regulation of glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; regulation of pentose-phosphate shunt; gluconeogenesis

Research Articles on PGAM1

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Product Notes

The PGAM1 pgam1 (Catalog #AAA3008562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGAM1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PGAM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PGAM1 pgam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGAM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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