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Western Blot (WB) (Western blot analysis of PFTK1 Antibody (N-term P82) in mouse cerebellum tissue lysates (35ug/lane). PFTK1 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse PFTK1 Polyclonal Antibody | anti-PFTK1 antibody

PFTK1, NT (Cell Division Protein Kinase 14, Cyclin-dependent Kinase 14, CDK14, KIAA0834, PFTAIRE1, hPFTAIRE1, Serine/threonine Protein Kinase PFTAIRE-1) (MaxLight 550)

Gene Names
CDK14; PFTK1; PFTAIRE1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
PFTK1; Polyclonal Antibody; NT (Cell Division Protein Kinase 14; Cyclin-dependent Kinase 14; CDK14; KIAA0834; PFTAIRE1; hPFTAIRE1; Serine/threonine Protein Kinase PFTAIRE-1) (MaxLight 550); EC=2.7.11.22; anti-PFTK1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PFTK1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-PFTK1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human PFTK1 (KLH).
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PFTK1 Antibody (N-term P82) in mouse cerebellum tissue lysates (35ug/lane). PFTK1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PFTK1 Antibody (N-term P82) in mouse cerebellum tissue lysates (35ug/lane). PFTK1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PFTK1 Antibody (N-term P82) (RB18842) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PFTK1 Antibody (N-term P82) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PFTK1 Antibody (N-term P82) (RB18842) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PFTK1 Antibody (N-term P82) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PFTK1 Antibody (N-term P82) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (PFTK1 Antibody (N-term P82) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-PFTK1 antibody
References
1. Denoeud, F., et.al., Genome Res. 17(6), 746-759 (2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,760 Da
NCBI Official Full Name
cyclin-dependent kinase 14 isoform b
NCBI Official Synonym Full Names
cyclin dependent kinase 14
NCBI Official Symbol
CDK14
NCBI Official Synonym Symbols
PFTK1; PFTAIRE1
NCBI Protein Information
cyclin-dependent kinase 14
UniProt Protein Name
Cyclin-dependent kinase 14
UniProt Gene Name
CDK14
UniProt Synonym Gene Names
KIAA0834; PFTK1; hPFTAIRE1
UniProt Entry Name
CDK14_HUMAN

NCBI Description

PFTK1 is a member of the CDC2 (MIM 116940)-related protein kinase family (Yang and Chen, 2001 [PubMed 11313143]).[supplied by OMIM, Mar 2008]

Uniprot Description

CDK14: a protein kinase of the CDK family. May play a role in meiosis as well as in neuron differentiation and/or function. Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Two alternatively spliced isoforms have been described.

Protein type: Cell cycle regulation; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; Motility/polarity/chemotaxis; Kinase, protein; CMGC group; CDK family; CDK/TAIRE subfamily; TAIRE subfamily

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; cyclin binding; cyclin-dependent protein kinase activity; ATP binding

Biological Process: Wnt receptor signaling pathway; regulation of cell cycle; cell division; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation

Research Articles on PFTK1

Similar Products

Product Notes

The PFTK1 cdk14 (Catalog #AAA6492797) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFTK1, NT (Cell Division Protein Kinase 14, Cyclin-dependent Kinase 14, CDK14, KIAA0834, PFTAIRE1, hPFTAIRE1, Serine/threonine Protein Kinase PFTAIRE-1) (MaxLight 550) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PFTK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PFTK1 cdk14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFTK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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