<
Western Blot (WB)
(Anti-PFN1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:15 kDa Observed band size:15 kDa)
Rabbit PFN1/Profilin 1 Polyclonal Antibody | anti-PFN1 antibody
Anti-PFN1/Profilin 1 Antibody, Rabbit Polyclonal
Gene Names
Pfn1; Pfn
Reactivity
Mouse, RatPredicted to React with: Bovin (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
PFN1/Profilin 1; Polyclonal Antibody; Anti-PFN1/Profilin 1 Antibody; Rabbit Polyclonal; PFN1/Profilin 1 Antibody; Rabbit PAb; Antigen Affinity Purified; profilin 1; Anti-Pfn Antibody; anti-PFN1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Predicted to React with: Bovin (Species predicted to react based on 100% sequence homology)
Predicted to React with: Bovin (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse PFN1/Profilin 1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PFN1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:200-1:1000
IHC-P: 1:200-1:1000
Immunogen
A synthetic peptide corresponding to the center region of the Mouse PFN1/Profilin 1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse PFN1/Profilin 1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PFN1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:15 kDa Observed band size:15 kDa)
Western Blot (WB)
(Anti-PFN1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse spleen tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:15 kDa Observed band size:15 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining PFN1 in mouse kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining PFN1 in mouse kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining PFN1 in mouse colon with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining PFN1 in mouse colon with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining PFN1 in rat kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining PFN1 in rat kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-PFN1 antibody
Mutations in the profilin 1 (PFN1) gene cause amyotrophic lateral sclerosis (ALS), a neurodegenerative disease caused by the loss of motor neurons leading to paralysis and eventually death. PFN1 is a small actin-binding protein that promotes formin-based actin polymerization and regulates numerous cellular functions.
Product Categories/Family for anti-PFN1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,957 Da
NCBI Official Full Name
profilin-1
NCBI Official Synonym Full Names
profilin 1
NCBI Official Symbol
Pfn1
NCBI Official Synonym Symbols
Pfn
NCBI Protein Information
profilin-1; profilin I; actin binding protein
UniProt Protein Name
Profilin-1
UniProt Gene Name
Pfn1
UniProt Entry Name
PROF1_MOUSE
Similar Products
Product Notes
The PFN1 pfn1 (Catalog #AAA8103753) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PFN1/Profilin 1 Antibody, Rabbit Polyclonal reacts with Mouse, Rat Predicted to React with: Bovin (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's PFN1/Profilin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:200-1:1000. Researchers should empirically determine the suitability of the PFN1 pfn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFN1/Profilin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
