Rabbit PFLB Polyclonal Antibody | anti-PFLB antibody

Rabbit anti-Escherichia coli (strain K12) PFLB Polyclonal Antibody

Gene Names

pflB; ECK0894; JW0886; pfl

Reactivity

E Coli (strain K12)

Applications

ELISA, Western Blot

Purity

Protein A/G

Synonyms

PFLB; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) PFLB Polyclonal Antibody; PFLB Antibody; Formate acetyltransferase 1; EC 2.3.1.54; Pyruvate formate-lyase 1; pflB pfl b0903 JW0886; anti-PFLB antibody

Ordering

Host

Rabbit

Reactivity

E Coli (strain K12)

Clonality

Polyclonal

Isotype

IgG

Purity/Purification

Protein A/G

Form/Format

Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4

Applicable Applications for anti-PFLB antibody

ELISA (EIA), WB (Western Blot) (ensure identification of antigen)

Immunogen

Recombinant Escherichia coli (strain K12) pflB protein

Conjugation

Non-conjugate

Modification

Unmodified

Deliverables

1. 200ug recombinant immunogen protein/peptide (positive control).
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.

Quality Guarantee

1. Antibody purity >90% confirmed by SDS-PAGE.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).

Complementary Antibody

Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:
* 6*His Monoclonal Antibody #MBS1491670
* DYKDDDDK tag Monoclonal Antibody #MBS1493946
* GAPDH Monoclonal Antibody #MBS1495524
* GFP Monoclonal Antibody #MBS1496050
* Myc tag Monoclonal Antibody #MBS1497694
* GST Monoclonal Antibody #MBS1497871
* Sumo tag Monoclonal Antibody #MBS1498878
* Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510
* HA-Tag Monoclonal Antibody #MBS7135631
* E-Tag Monoclonal Antibody #MBS7135632
* TUBA1A Monoclonal Antibody #MBS7135645
* TUBB Monoclonal Antibody #MBS9018332

Preparation and Storage

Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

NCBI GI #

16128870

NCBI GeneID

945514

NCBI Accession #

NP_415423.1

NCBI GenBank Nucleotide #

NC_000913.3

UniProt Accession #

P09373; P76826; Q47478

Molecular Weight

85,357 Da

NCBI Official Full Name

formate C-acetyltransferase 1, anaerobic; pyruvate formate-lyase 1

NCBI Official Symbol

pflB

NCBI Official Synonym Symbols

ECK0894; JW0886; pfl

NCBI Protein Information

formate C-acetyltransferase 1, anaerobic; pyruvate formate-lyase 1

UniProt Protein Name

Formate acetyltransferase 1

Protein Family

Formate acetyltransferase

UniProt Gene Name

pflB

UniProt Synonym Gene Names

pfl

NCBI Description

HT_Cmplx8_Cyt: [More information is available at EcoGene: EG10701].

Uniprot Description

MiscellaneousSeveral mechanisms have been proposed based on complexes formed with substrate analogs. After activation by the glycine radical, the cysteine radical, Cys-420, can abstract hydrogen atoms from the other active site cysteine, Cys-419, and from coenzyme A, and it can also transfer hydrogen atoms to product radicals. The other active site cysteine can attack the central carbonyl of pyruvate and covalently bind the product acetyl group.

Research Articles on PFLB

1. FocA has a strong preference for formate as a substrate in vivo and not other acidic fermentation products and the tight coupling between FocA and PflB synthesis ensures adequate substrate delivery to the appropriate formate dehydrogenase.

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Product Notes

The PFLB pflb (Catalog #AAA7162473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Escherichia coli (strain K12) PFLB Polyclonal Antibody reacts with E Coli (strain K12) and may cross-react with other species as described in the data sheet. AAA Biotech's PFLB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the PFLB pflb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFLB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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