Western Blot (WB)
(PET112L Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PET112L antibody detected the PET112L protein (arrow).)
Rabbit anti-Human PET112L Polyclonal Antibody | anti-PET112L antibody
PET112L, NT (PET112, PET112L, Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial, Cytochrome oxidase assembly factor PET112 homolog) (PE)
Gene Names
GATB; PET112; HSPC199; PET112L
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PET112L; Polyclonal Antibody; NT (PET112; Glutamyl-tRNA(Gln) amidotransferase subunit B; mitochondrial; Cytochrome oxidase assembly factor PET112 homolog) (PE); PET112; Cytochrome oxidase assembly factor PET112 homolog; anti-PET112L antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PET112L antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PET112L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human PET112L.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PET112L Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PET112L antibody detected the PET112L protein (arrow).)
Western Blot (WB)
(PET112L Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PET112L antibody detected the PET112L protein (arrow).)
Related Product Information for anti-PET112L antibody
PET112L furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (Potential).
Product Categories/Family for anti-PET112L antibody
References
Nagao, A., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16209-16214(2009). Petruzzella, V., et al. Genomics 54(3):494-504(1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,864 Da
NCBI Official Full Name
glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
NCBI Official Synonym Full Names
glutamyl-tRNA amidotransferase subunit B
NCBI Official Symbol
GATB
NCBI Official Synonym Symbols
PET112; HSPC199; PET112L
NCBI Protein Information
glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
UniProt Protein Name
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
UniProt Gene Name
GATB
UniProt Synonym Gene Names
Glu-AdT subunit B
Uniprot Description
PET112L: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). Belongs to the GatB/GatE family. GatB subfamily.
Protein type: EC 6.3.5.-; Ligase; Mitochondrial; RNA-binding; Translation
Chromosomal Location of Human Ortholog: 4q31.3
Cellular Component: mitochondrion
Molecular Function: ATP binding; glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; translation factor activity, nucleic acid binding
Biological Process: mitochondrial translation; translation
Research Articles on PET112L
Similar Products
Product Notes
The PET112L gatb (Catalog #AAA6332748) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PET112L, NT (PET112, PET112L, Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial, Cytochrome oxidase assembly factor PET112 homolog) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PET112L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PET112L gatb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PET112L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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