Western Blot (WB)
((0.5 ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Perilipin Polyclonal Antibody | anti-PLIN antibody
Goat anti-Perilipin (C Terminus) Antibody
Expected from sequence similarity: Human, Rat, Dog
Expected from sequence similarity: Human, Rat, Dog
Western Blot: Approx 55kDa band observed in Human Adipose lysates (calculated MW of 56. 0kDa according to NP_002657.2). Recommended concentration: 0.01-0.03 ug/ml. Primary incubation 1 hour at room temperature.
Western Blot (WB)
((0.5 ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5 ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunofluorescence (IF)
(Staining of differentiated 3T3-L1 adipocytes. Data kindly provided by Prof. J. Granneman, Detroit, USA)
Immunofluorescence (IF)
(Staining of differentiated 3T3-L1 adipocytes. Data kindly provided by Prof. J. Granneman, Detroit, USA)
Immunohistochemistry
((4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry
((4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]
Uniprot Description
perilipin 1: an adipocyte protein of the peripilin family. A modulator of adipocyte lipid metabolism, coating lipid storage droplets to protect them from being broken down by hormone-sensitive lipase. A major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 15q26
Cellular Component: endoplasmic reticulum; lipid particle
Molecular Function: lipid binding
Biological Process: lipid metabolic process
Disease: Lipodystrophy, Familial Partial, Type 4
Research Articles on PLIN
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Product Notes
The PLIN plin1 (Catalog #AAA420598) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Perilipin (C Terminus) Antibody reacts with Tested: Human Expected from sequence similarity: Human, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Perilipin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Imuunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approx 55kDa band observed in Human Adipose lysates (calculated MW of 56. 0kDa according to NP_002657.2). Recommended concentration: 0.01-0.03 ug/ml. Primary incubation 1 hour at room temperature. Researchers should empirically determine the suitability of the PLIN plin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Perilipin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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