Goat PERICENTRIN 1 Polyclonal Antibody | anti-NUP85 antibody

GOAT ANTI HUMAN PERICENTRIN 1 (C-TERMINAL)

Cat. Num. AAA221385

• Gene Names: NUP85; Nup75; FROUNT
• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: PERICENTRIN 1; Polyclonal Antibody; GOAT ANTI HUMAN PERICENTRIN 1 (C-TERMINAL); anti-NUP85 antibody

• Host: Goat
• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises the C-terminal of human pericentrin 1, a 75 kDa component of the filamentous matrix of the centrosome, expressed in all tissues. When associated with centrosome and Golgi localised protein kinase N-associated protein (CG-NAP), pericentrin 1 anchors the gamma-tubulin ring complex, providing a site for microtubule nucleation and the initial establishment of organized microtubule arrays. It binds calmodulin and is a constituent of nuclear pore proteins (nup) 107-160, a sub-complex of the nuclear pore complex. Pericentrin 1 is thought to play a role in the functioning of the centrosomes, cytoskeleton, and cell-cycle progression.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 656

• Applicable Applications for anti-NUP85 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 75kDa in Hela cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.; ELISA: Maximum Dilution: >1/32,000; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 3ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide; 0.5% Bovine Serum Albumin
• Immunogen: Peptide sequence C-ARAIIREGSLEGS corresponding to the C-terminal of pericentrin 1 (NP_079120).; Buffer Solution
• Antiserum Preparation: Antisera to human pericentrin 1 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-NUP85 antibody

MBS221385 recognises the C-terminal of human pericentrin 1, a 75 kDa component of the filamentous matrix of the centrosome, expressed in all tissues. When associated with centrosome and Golgi localised protein kinase N-associated protein (CG-NAP), pericentrin 1 anchors the gamma-tubulin ring complex, providing a site for microtubule nucleation and the initial establishment of organized microtubule arrays. It binds calmodulin and is a constituent of nuclear pore proteins (nup) 107-160, a sub-complex of the nuclear pore complex. Pericentrin 1 is thought to play a role in the functioning of the centrosomes, cytoskeleton, and cell-cycle progression.

NCBI and Uniprot Product Information

• NCBI GI #: 13376259
• NCBI GeneID: 79902
• NCBI Accession #: NP_079120.1
• NCBI GenBank Nucleotide #: NM_024844.4
• UniProt Accession #: Q9BW27; Q8NDI4; Q9H9U1; B4DMQ3; B4DPW1
• Molecular Weight: 69,800 Da
• NCBI Official Full Name: nuclear pore complex protein Nup85 isoform 1
• NCBI Official Synonym Full Names: nucleoporin 85kDa
• NCBI Official Symbol: NUP85
• NCBI Official Synonym Symbols: Nup75; FROUNT
• NCBI Protein Information: nuclear pore complex protein Nup85; nucleoporin Nup75; nucleoporin Nup85
• UniProt Protein Name: Nuclear pore complex protein Nup85
• Protein Family: Nucleoporin
• UniProt Gene Name: NUP85
• UniProt Synonym Gene Names: NUP75; PCNT1
• UniProt Entry Name: NUP85_HUMAN

NCBI Description

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

NUP85: Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade. Belongs to the nucleoporin Nup85 family.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: kinetochore; nuclear membrane; membrane; spindle; nuclear envelope; cytosol

Molecular Function: protein binding

Biological Process: lamellipodium biogenesis; mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; viral infectious cycle; glucose transport; macrophage chemotaxis; protein transport; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport