<
Western Blot (WB)
(Western blot analysis of anti-PCK2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human PEPCK2 Polyclonal Antibody | anti-PEPCK2 antibody
PEPCK2, NT (Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial), PEPCK-2, PEPCK, PCK2, Mitochondrial Phosphoenolpyruvate Carboxykinase 2, Phosphoenolpyruvate Carboxykinase [GTP] Mitochondrial, PEPCK-M, PEP Carboxykinase, Phosphoenolpyruvate Carboxylase) (
Gene Names
PCK2; PEPCK; PEPCK2; PEPCK-M
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
PEPCK2; Polyclonal Antibody; NT (Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial); PEPCK-2; PEPCK; PCK2; Mitochondrial Phosphoenolpyruvate Carboxykinase 2; Phosphoenolpyruvate Carboxykinase [GTP] Mitochondrial; PEPCK-M; PEP Carboxykinase; Phosphoenolpyruvate Carboxylase) (; EC=4.1.1.32; GTP Mitochondrial Precursor; HGNC:8725; anti-PEPCK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PCK2.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-PEPCK2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human PCK2 (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-PCK2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-PCK2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.)
Western Blot (WB)
(Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PCK2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PCK2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-PEPCK2 antibody
References
1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Modaressi, S., et al., Biochem. J. 333(Pt 2), 359-366 (1998). 3. Modaressi, S., et al., Biochem. J. 315(Pt 3), 807-814 (1996).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,564 Da
NCBI Official Full Name
phosphoenolpyruvate carboxykinase
NCBI Official Synonym Full Names
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
NCBI Official Symbol
PCK2
NCBI Official Synonym Symbols
PEPCK; PEPCK2; PEPCK-M
NCBI Protein Information
phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PEP carboxykinase; phosphopyruvate carboxylase
UniProt Protein Name
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Protein Family
UniProt Gene Name
PCK2
UniProt Synonym Gene Names
PEPCK2; PEPCK-M
UniProt Entry Name
PCKGM_HUMAN
Similar Products
Product Notes
The PEPCK2 pck2 (Catalog #AAA6492659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PEPCK2, NT (Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial), PEPCK-2, PEPCK, PCK2, Mitochondrial Phosphoenolpyruvate Carboxykinase 2, Phosphoenolpyruvate Carboxykinase [GTP] Mitochondrial, PEPCK-M, PEP Carboxykinase, Phosphoenolpyruvate Carboxylase) ( reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PEPCK2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PEPCK2 pck2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEPCK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
