Rabbit Penicillinase, E. clocae Polyclonal Antibody | anti-blaI antibody
Penicillinase, E. clocae (Biotin)
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Highly PurifiedPuri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
Synonyms
Penicillinase; E. clocae; Polyclonal Antibody; E. clocae (Biotin); Anti -Penicillinase; anti-blaI antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as puri ed and partially puri ed Penicillinase [Enterobacter cloacae].
Purity/Purification
Highly Purified
Puri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
Puri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, before the addition of glycerol to 40%. Labeled with biotin.
Applicable Applications for anti-blaI antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence as well as other antibody based assays using streptavidin or avidin conjugates.
Dilution: ELISA: 1:2000 to 1:10,000. 1ug of Penicillinase was used in a standard capture ELISA with ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
Dilution: ELISA: 1:2000 to 1:10,000. 1ug of Penicillinase was used in a standard capture ELISA with ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
Immunogen
Penicillinase [Enterobacter cloacae]
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-blaI antibody
Penicillinase is a specific type of -lactamase, showing specificity for penicillins, again by hydrolysing the beta-lactam ring. Molecular weights of the various penicillinases tend to cluster near 50kD. Penicillinase was the first -lactamase to be identified: It was first isolated by Abraham and Chain in 1940 from Gram-negative E. coli even before penicillin entered clinical use,[2] but penicillinase production quickly spread to bacteria that previously did not produce it or produced it only rarely. Penicillinase-resistant beta-lactams such as methicillin were developed, but there is now widespread resistance to even these. Penicillinases are classified as Group 2 along with cephalosporinases, both are inhibited by clavulanic acid, corresponding to the molecular classes A and D reflecting the original TEM and SHV genes. Due to the increasing number of TEM- and SHV-derived -lactamases, Penicillinases are further divided into the subclass 2a.
Product Categories/Family for anti-blaI antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
penicillinase repressor
NCBI Official Symbol
blaI
NCBI Protein Information
penicillinase repressor
Protein Family
Similar Products
Product Notes
The blaI (Catalog #AAA6007400) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Penicillinase, E. clocae can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence as well as other antibody based assays using streptavidin or avidin conjugates. Dilution: ELISA: 1:2000 to 1:10,000. 1ug of Penicillinase was used in a standard capture ELISA with ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT. Researchers should empirically determine the suitability of the blaI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Penicillinase, E. clocae, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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