Rabbit anti-Human, Mouse Pellino-1 Polyclonal Antibody | anti-PELI1 antibody

Pellino-1 Antibody

Cat. Num. AAA9629048

• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Pellino-1; Polyclonal Antibody; Pellino-1 Antibody; E3 ubiquitin-protein ligase pellino homolog 1; PELI1; PELI1_HUMAN; Pellino E3 ubiquitin protein ligase 1; Pellino homolog 1; Pellino protein; Pellino-related intracellular-signaling molecule; Pellino1; PRISM; Protein pellino homolog 1; anti-PELI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Pellino-1 Antibody detects endogenous levels of total Pellino-1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PELI1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the pellino family.
• Post Translational Modifications: Phosphorylation by IRAK1 and IRAK4 enhances its E3 ligase activity.Sumoylated.
• Subunit Structure: Interacts with MAP3K7. Upon IL1B treatment, forms a complex with TRAF6, IRAK1, IRAK4 and MYD88; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents the complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression. Interacts (via atypical FHA domain) with RIPK3; preferentially binds to the 'Thr-182' phosphorylated form of RIPK3. Interacts with RIPK1 and IRAK1.
• Tissue Specificity: Expressed at high levels in normal skin but decreased in keratinocytes from toxic epidermal necrolysis (TEN) patients (at protein level).
• Function: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3. Negatively regulates necroptosis by reducing RIPK3 expression. Mediates 'Lys-63'-linked ubiquitination of RIPK1.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PELI1 antibody

Primary

NCBI and Uniprot Product Information

• NCBI GI #: 11037063
• NCBI GeneID: 57162
• NCBI Accession #: NP_065702
• NCBI GenBank Nucleotide #: NM_020651.3
• UniProt Accession #: Q96FA3; Q96SM0; Q9GZY5; Q9HCX0
• Molecular Weight: 46 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase pellino homolog 1
• NCBI Official Synonym Full Names: pellino E3 ubiquitin protein ligase 1
• NCBI Official Symbol: PELI1
• NCBI Protein Information: E3 ubiquitin-protein ligase pellino homolog 1; pellino-1; protein pellino homolog 1; pellino-related intracellular-signaling molecule
• UniProt Protein Name: E3 ubiquitin-protein ligase pellino homolog 1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: PELI1
• UniProt Synonym Gene Names: PRISM; Pellino-1
• UniProt Entry Name: PELI1_HUMAN

Uniprot Description

PELI1: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. Found in a complex containing TRAF6, IRAK1 and IRAK4. Interacts with MAP3K7. Belongs to the pellino family.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Adaptor/scaffold; Ubiquitin conjugating system; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: cytosol; nucleus

Molecular Function: protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: positive regulation of cytokine production; positive regulation of toll-like receptor 3 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; response to dsRNA; positive regulation of toll-like receptor 4 signaling pathway; response to lipopolysaccharide; Toll signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of T cell proliferation; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; positive regulation of B cell proliferation; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway