Rabbit anti-Human PEG10 Polyclonal Antibody | anti-PEG10 antibody

PEG10, NT (PEG10, EDR, KIAA1051, MAR2, MART2, MEF3L1, RGAG3, Retrotransposon-derived protein PEG10, Embryonal carcinoma differentiation-regulated protein, Mammalian retrotransposon-derived protein 2, Myelin expression factor 3-like protein 1, Paternally e

Cat. Num. AAA6332636

• Gene Names: PEG10; EDR; HB-1; Mar2; RTL2; MEF3L; Mart2; RGAG3; SIRH1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PEG10; Polyclonal Antibody; NT (PEG10; EDR; KIAA1051; MAR2; MART2; MEF3L1; RGAG3; Retrotransposon-derived protein PEG10; Embryonal carcinoma differentiation-regulated protein; Mammalian retrotransposon-derived protein 2; Myelin expression factor 3-like protein 1; Paternally e; Paternally expressed gene 10 protein; Retrotransposon gag domain-containing protein 3; Retrotransposon-derived gag-like polyprotein; Ty3/Gypsy-like protein; anti-PEG10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
• Sequence Length: 708

• Applicable Applications for anti-PEG10 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PEG10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PEG10.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-PEG10 antibody

This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs), RF1 and RF1/RF2, as well as retroviral-like slippage and pseudoknot elements, which can induce a-1 nucleotide frame-shift. ORF1 encodes a shorter isoform with a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons. The longer isoform is the result of-1 translational frame-shifting leading to translation of a gag/pol-like protein combining RF1 and RF2. It contains the active-site consensus sequence of the protease domain of pol proteins. Additional isoforms resulting from alternatively spliced transcript variants, as well as from use of upstream non-AUG (CUG) start codon, have been reported for this gene. Increased expression of this gene is associated with hepatocellular carcinomas.

Product Categories/Family for anti-PEG10 antibody

Antibodies; Cancer Markers

References

Tsuji, K., et al. Cancer Genet. Cytogenet. 198(2):118-125(2010). Chang, Y., et al. Zhonghua Gan Zang Bing Za Zhi 18(4):288-291(2010). Lux, H., et al. PLoS ONE 5 (1), E8686 (2010): Wang, C., et al. FEBS Lett. 582(18):2793-2798(2008). Lux, A., et al. J. Biol. Chem. 280(9):8482-8493(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 94421475
• NCBI GeneID: 23089
• NCBI Accession #: NP_055883.2; NP_001035242.1; NP_001171891.1; NP_001171890.1
• NCBI GenBank Nucleotide #: NP_055883.2
• UniProt Accession #: Q86TG7
• NCBI Official Full Name: retrotransposon-derived protein PEG10 isoform 1
• NCBI Official Synonym Full Names: paternally expressed 10
• NCBI Official Symbol: PEG10
• NCBI Official Synonym Symbols: EDR; HB-1; Mar2; RTL2; MEF3L; Mart2; RGAG3; SIRH1
• NCBI Protein Information: retrotransposon-derived protein PEG10
• UniProt Protein Name: Retrotransposon-derived protein PEG10
• Protein Family: Retrotransposon-derived protein
• UniProt Gene Name: PEG10
• UniProt Synonym Gene Names: EDR; KIAA1051; MAR2; MART2; MEF3L1; RGAG3; MEF3-like protein 1
• UniProt Entry Name: PEG10_HUMAN

NCBI Description

This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

PEG10: Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation. May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter. Up-regulated by MYC and dihydrotestosteron (DHT). Interacts with ALK1, SIAH1 and SIAH2. 2 isoforms of the human protein are produced by ribosomal frameshifting.

Protein type: RNA-binding; Cell cycle regulation; Apoptosis; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding

Biological Process: apoptosis; cell differentiation; negative regulation of transforming growth factor beta receptor signaling pathway