Rabbit anti-Human PEF1 Polyclonal Antibody | anti-PEF1 antibody

PEF1, ID (PEF1, ABP32, Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1) (FITC)

Cat. Num. AAA6332620

• Gene Names: PEF1; ABP32; PEF1A
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PEF1; Polyclonal Antibody; ID (PEF1; ABP32; Peflin; PEF protein with a long N-terminal hydrophobic domain; Penta-EF hand domain-containing protein 1) (FITC); Penta-EF hand domain-containing protein 1; anti-PEF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PEF1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-121 amino acids from the Central region of human PEF1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PEF1 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PEF1 antibody detected the PEF1 protein (arrow).)

Related Product Information for anti-PEF1 antibody

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.

Product Categories/Family for anti-PEF1 antibody

Antibodies; Proteins

References

Lamesch, P., et al. Genomics 89(3):307-315(2007). Hansen, C., et al. FEBS Lett. 545 (2-3), 151-154 (2003): Satoh, H., et al. Biochim. Biophys. Acta 1600 (1-2), 61-67 (2002): Kitaura, Y., et al. Arch. Biochem. Biophys. 399(1):12-18(2002). Kitaura, Y., et al. J. Biol. Chem. 276(17):14053-14058(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 6912582
• NCBI GeneID: 553115
• NCBI Accession #: NP_036524.1
• NCBI GenBank Nucleotide #: NM_012392.3
• UniProt Accession #: Q9UBV8
• Molecular Weight: 30,381 Da
• NCBI Official Full Name: peflin
• NCBI Official Synonym Full Names: penta-EF-hand domain containing 1
• NCBI Official Symbol: PEF1
• NCBI Official Synonym Symbols: ABP32; PEF1A
• NCBI Protein Information: peflin; PEF protein with a long N-terminal hydrophobic domain; penta-EF hand domain containing 1
• UniProt Protein Name: Peflin
• Protein Family: Peflin
• UniProt Gene Name: PEF1
• UniProt Synonym Gene Names: ABP32
• UniProt Entry Name: PEF1_HUMAN

NCBI Description

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.[provided by RefSeq, May 2010]

Uniprot Description

PEF1: Heterodimer; heterodimerizes with PDCD6/ALG2. Dissociates from PDCD6/ALG2 in presence of Ca(2+).

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: membrane; cytoplasm

Molecular Function: protein dimerization activity; protein binding; protein heterodimerization activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding

Biological Process: proteolysis; response to calcium ion