Western Blot (WB)
(Western blot analysis of PECI Antibody (C-term) in HepG2 cell line lysates (35ug/lane).PECI (arrow) was detected using the purified Pab.)
Rabbit anti-Human PECI Polyclonal Antibody | anti-ECI2 antibody
PECI Antibody (C-term)
Western Blot (WB)
(Western blot analysis of PECI Antibody (C-term) in HepG2 cell line lysates (35ug/lane).PECI (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PECI Antibody (C-term) in HepG2 cell line lysates (35ug/lane).PECI (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PECI Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PECI Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(Western blot analysis of PECI (arrow) using rabbit polyclonal PECI Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PECI gene.)
Western Blot (WB)
(Western blot analysis of PECI (arrow) using rabbit polyclonal PECI Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PECI gene.)
Suk,K., et.al., Biochim. Biophys. Acta 1454 (1), 126-131 (1999)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2011]
Uniprot Description
PECI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 5.3.3.8; Lipid Metabolism - fatty acid; Isomerase
Chromosomal Location of Human Ortholog: 6p24.3
Cellular Component: nucleoplasm; peroxisomal matrix; intracellular membrane-bound organelle; mitochondrion; membrane
Molecular Function: acyl-CoA binding; dodecenoyl-CoA delta-isomerase activity; receptor binding
Biological Process: metabolic process; fatty acid catabolic process
Research Articles on ECI2
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Product Notes
The ECI2 eci2 (Catalog #AAA9204475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 331-358. The PECI Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PECI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ECI2 eci2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PECI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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