Rabbit PEBP1 Polyclonal Antibody | anti-PEBP1 antibody

Anti-PEBP1 Antibody

Cat. Num. AAA419452

• Gene Names: PEBP1; PBP; PEBP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: PEBP1; Polyclonal Antibody; Anti-PEBP1 Antibody; anti-PEBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using PEBP1 antibody. Immunohistochemistry of paraffin-embedded human thyroid cancer using PEBP1 antibody at dilution of 1:200 (400x lens). Immunohistochemistry of paraffin-embedded rat liver using PEBP1 antibody at dilution of 1:200 (200x lens). Immunofluorescence analysis of HeLa cell using PEBP1 antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 187

• Applicable Applications for anti-PEBP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:50- 1:200
• Immunogen: Recombinant protein of human PEBP1
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Related Product Information for anti-PEBP1 antibody

Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-B pathway, including NF-B-inducing kinase (NIK), TAK1, IKK+/- and IKK2 (5). These interactions antagonize cytokine-induced activation of the NF-B pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6).

NCBI and Uniprot Product Information

• NCBI GI #: 75812940
• NCBI GeneID: 431786
• NCBI Accession #: NP_001028795.1
• NCBI GenBank Nucleotide #: NM_001033623.2
• UniProt Accession #: P13696; Q3T0H9
• Molecular Weight: 20,986 Da
• NCBI Official Full Name: phosphatidylethanolamine-binding protein 1
• NCBI Official Symbol: PEBP1
• NCBI Official Synonym Symbols: PBP; PEBP
• NCBI Protein Information: phosphatidylethanolamine-binding protein 1
• UniProt Protein Name: Phosphatidylethanolamine-binding protein 1
• Protein Family: Phosphatidylethanolamine-binding protein
• UniProt Gene Name: PEBP1
• UniProt Synonym Gene Names: PBP; PEBP; PEBP-1; HCNP

Uniprot Description

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation ().