Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human ATP-dependent RNA helicase DHX8 (DHX8) Standard Curve.)

Human ATP-Dependent RNA Helicase DHX8 (DHX8) ELISA Kit | DHX8 elisa kit

Human ATP-Dependent RNA Helicase DHX8 (DHX8) ELISA Kit

Gene Names
DHX8; DDX8; Dhr2; HRH1; PRP22; PRPF22
Reactivity
Human
Synonyms
ATP-Dependent RNA Helicase DHX8 (DHX8); Human ATP-Dependent RNA Helicase DHX8 (DHX8) ELISA Kit; DHX8; DDX8; HRH1; PRP22; PRPF22; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase); DEAH-box protein 8; DHX8 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human ATP-dependent RNA helicase DHX8 (DHX8) ELISA Kit has high sensitivity and excellent specificity for detection of Human DHX8. No significant cross-reactivity or interference between Human DHX8 and analogues was observed.
Sequence Length
1220
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human ATP-dependent RNA helicase DHX8 (DHX8) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human ATP-dependent RNA helicase DHX8 (DHX8) Standard Curve.)
Related Product Information for DHX8 elisa kit
Description: This Human ATP-dependent RNA helicase DHX8 (DHX8) ELISA Kit employs a two-site sandwich ELISA to quantitate DHX8 in samples. An antibody specific for DHX8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHX8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHX8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHX8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: DHX8 is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ATP-dependent RNA helicase DHX8 isoform 1
NCBI Official Synonym Full Names
DEAH-box helicase 8
NCBI Official Symbol
DHX8
NCBI Official Synonym Symbols
DDX8; Dhr2; HRH1; PRP22; PRPF22
NCBI Protein Information
ATP-dependent RNA helicase DHX8
UniProt Protein Name
ATP-dependent RNA helicase DHX8
UniProt Gene Name
DHX8
UniProt Synonym Gene Names
DDX8
UniProt Entry Name
DHX8_HUMAN

NCBI Description

This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

DDX8: Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.

Protein type: RNA-binding; RNA processing; RNA splicing; EC 3.6.4.13; Helicase; Spliceosome

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing

Research Articles on DHX8

Similar Products

Product Notes

The Human DHX8 dhx8 (Catalog #AAA9715726) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715726 ELISA Kit recognizes Human DHX8. It is sometimes possible for the material contained within the vial of "ATP-Dependent RNA Helicase DHX8 (DHX8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.