SDS-Page
(Detection of PDX-1 with MBS619404. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-PDX-1.)
Goat anti-Mouse, Rat Pdx1 Polyclonal Antibody | anti-PDX1 antibody
Pdx1 (Pdx Family Homeodomain Transcription Factor, Pancreas/Duodenum Homeobox 1, Glucose Sensitive Factor, GSF, Insulin Promoter Factor 1, IPF1, Islet/Duodenum Homeobox 1, IDX1, Insulin Upstream Factor 1, IUF1, MODY4, Somatostatin Transactivating Factor 1
Reactivity
Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Pdx1; Polyclonal Antibody; Pdx1 (Pdx Family Homeodomain Transcription Factor; Pancreas/Duodenum Homeobox 1; Glucose Sensitive Factor; GSF; Insulin Promoter Factor 1; IPF1; Islet/Duodenum Homeobox 1; IDX1; Insulin Upstream Factor 1; IUF1; MODY4; Somatostatin Transactivating Factor 1; Anti -Pdx1 (Pdx Family Homeodomain Transcription Factor; anti-PDX1 antibody
Host
Goat
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Pdx1. Species Crossreactivity: rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-PDX1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Immunocytochemistry, and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunocytochemistry: 10ug/ml
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunocytochemistry: 10ug/ml
Immunogen
Purified, E. coli-derived recombinant mouse Pancrease/Duodenum Homeobox-1 (aa1-284; Accession # P52946).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of PDX-1 with MBS619404. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-PDX-1.)
SDS-Page
(Detection of PDX-1 with MBS619404. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-PDX-1.)
Related Product Information for anti-PDX1 antibody
PDX-1 (Pancreas-duodenum homeobox 1; also IPF-1, IDX-1, STF-1, GSF and IUF-1) is a 42kD member of the IPF-1 subfamily, ANTP homeobox family of proteins. It is expressed in developing pancreas, plus pancreatic beta-cells, and duodenal crypt epithelium. In concert with HNF6, PDX-1 drives pancreatic endocrine cell formation by regulating the expression of neurogenin-3. In adult beta-cells, PDX-1 binds to PCIF-1 to regulate insulin production. Mouse PDX-1 is 284aa in length (Accession # P52946). It contains one transactivation domain (aa13-73) a PBX1 P-P-M-W-K dimerization motif (aa119-124), and a DNA-binding homeodomain with an embedded NLS (aa148-206). Full-length mouse PDX-1 shares 94% and 88% aa identity with rat and human PDX-1, respectively.
Product Categories/Family for anti-PDX1 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
45,362 Da
NCBI Official Full Name
PDX1
UniProt Protein Name
Pyruvate dehydrogenase complex protein X component, mitochondrial
Protein Family
UniProt Gene Name
PDX1
UniProt Entry Name
ODPX_YEAST
Uniprot Description
Function: Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
Subunit structure: Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.
Subcellular location: Mitochondrion matrix.
Miscellaneous: Present with 414 molecules/cell in log phase SD medium. Ref.7
Sequence similarities: Belongs to the 2-oxoacid dehydrogenase family.Contains 1 lipoyl-binding domain.
Similar Products
Product Notes
The Pdx1 pdx1 (Catalog #AAA619404) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Pdx1 (Pdx Family Homeodomain Transcription Factor, Pancreas/Duodenum Homeobox 1, Glucose Sensitive Factor, GSF, Insulin Promoter Factor 1, IPF1, Islet/Duodenum Homeobox 1, IDX1, Insulin Upstream Factor 1, IUF1, MODY4, Somatostatin Transactivating Factor 1 reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Pdx1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Dilution: ELISA: 0.5-1ug/ml Western Blot: 1ug/ml Immunocytochemistry: 10ug/ml. Researchers should empirically determine the suitability of the Pdx1 pdx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pdx1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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