Human Heat Shock Factor 1, HSF1 ELISA Kit | HSF1 elisa kit

Human Heat Shock Factor 1, HSF1 ELISA Kit

Cat. Num. AAA164303

• Gene Names: HSF1; HSTF1
• Reactivity: Human
• Synonyms: Heat Shock Factor 1; HSF1; Human Heat Shock Factor 1; HSF1 ELISA Kit; HSF1 elisa kit

• Reactivity: Human
• Sequence Length: 529

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.1ng/ml - 40ng/ml.
• Sensitivity: 0.065ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSF1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Heat Shock Factor 1 (also known as HSF1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human HSF1 antibody. HSF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human HSF1 Antibody is added and binds to HSF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human HSF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 5031767
• NCBI GeneID: 3297
• NCBI Accession #: NP_005517.1
• NCBI GenBank Nucleotide #: NM_005526.3
• UniProt Accession #: Q00613; Q53XT4; A8K4L0; A8MW26
• Molecular Weight: 52,881 Da
• NCBI Official Full Name: heat shock factor protein 1
• NCBI Official Synonym Full Names: heat shock transcription factor 1
• NCBI Official Symbol: HSF1
• NCBI Official Synonym Symbols: HSTF1
• NCBI Protein Information: heat shock factor protein 1
• UniProt Protein Name: Heat shock factor protein 1
• Protein Family: Heat shock factor protein
• UniProt Gene Name: HSF1
• UniProt Synonym Gene Names: HSTF1; HSTF 1

NCBI Description

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017]

Uniprot Description

Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed:11583998, PubMed:16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed:1986252, PubMed:26727489). Binds to chromatin at heat shock gene promoters (PubMed:25963659). Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed:9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed:14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed:17897941). Plays a role in the regulation of mitotic progression (PubMed:18794143). Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner (PubMed:26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed:26754925).