Immunofluorescence (IF)
Rabbit PDL1 Polyclonal Antibody | anti-PDL1 antibody
Anti PDL1 polyclonal antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
HumanOther species were not tested
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
PDL1; Polyclonal Antibody; Anti PDL1 polyclonal antibody; Programmed death ligand 1; PDCD1 ligand 1; CD274; hPD-L1; PDCD1L1; PD-L1; Programmed cell death 1 ligand 1; B7 homolog 1; PDCD1LG1; B7H1; B7-H1; anti-PDL1 antibody
Host
Rabbit
Reactivity
Human
Other species were not tested
Other species were not tested
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Form/Format
Liquid. Supplied as solution form in PBS, pH7.4, containing 0.05% proclin300, 50% glycerol.
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-PDL1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:2000-1:8000
IHC: 1:200-1:400
IHC: 1:200-1:400
Immunogen
Recombinant protein of human PDL1 (Phe19-Arg238)
Conjugation
Unconjugated
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 degree C for frequentUse. Store at -20 to -80 degree C for twelve months from the date of receipt.
Immunofluorescence (IF)
Immunofluorescence (IF)
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-PDL1 antibody
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33kDa, it is predicted to be 27-30kDa after signal peptide cleavage (PMID: 25609200, 17076679). The apparent molecular weight has also been reported as 45-70kDa, major glycosylated form of 45-50kDa and multiple post-translational modifications form of 65-70kDa (PMID: 18760278, 16493058).
Product Categories/Family for anti-PDL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,275 Da
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform b
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
Uniprot Description
CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p24
Cellular Component: plasma membrane; integral to membrane; endomembrane system; external side of plasma membrane
Molecular Function: protein binding
Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction
Research Articles on PDL1
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Product Notes
The PDL1 cd274 (Catalog #AAA1560328) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti PDL1 polyclonal antibody reacts with Human Other species were not tested and may cross-react with other species as described in the data sheet. AAA Biotech's PDL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:2000-1:8000 IHC: 1:200-1:400. Researchers should empirically determine the suitability of the PDL1 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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