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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Laminin subunit alpha-1 (LAMA1) Standard Curve.)

Mouse Laminin Subunit alpha-1 (LAMA1) ELISA Kit | LAMA1 elisa kit

Mouse Laminin Subunit alpha-1 (LAMA1) ELISA Kit

Gene Names
Lama1; Lama; AA408497
Reactivity
Mouse
Synonyms
Laminin Subunit alpha-1 (LAMA1); Mouse Laminin Subunit alpha-1 (LAMA1) ELISA Kit; LAMA1; LAMA; LAMA1 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Mouse Laminin subunit alpha-1 (LAMA1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LAMA1. No significant cross-reactivity or interference between Mouse LAMA1 and analogues was observed.
Sequence Length
3083
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Laminin subunit alpha-1 (LAMA1) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Laminin subunit alpha-1 (LAMA1) Standard Curve.)
Related Product Information for LAMA1 elisa kit
Description: This Mouse Laminin subunit alpha-1 (LAMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate LAMA1 in samples. An antibody specific for LAMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Laminin is a basement membrane protein composed of 3 nonidentical chains, A (400 kD), B1 (230 kD), and B2 (220 kD), which are arranged in a cross-shaped structure. Mattei et al. (1989) used in situ hybridization to localize the human laminin A locus to chromosome 18p11.31.By study of mouse-Chinese hamster somatic cell hybrids, Kaye et al. assigned the gene for laminin A to mouse chromosome 17 and confirmed the assignment of the gene for laminin B2 to mouse chromosome 1. Haaparanta et al. reported the nucleotide sequence of LAMA cDNA. They found an open reading frame encoding 3,075 amino acids. Nissinen et al. reported that the complete human laminin A chain contains a 17-amino acid signal peptide and a 3,058-residue A chain proper. The chain has a distinct domain structure with numerous internal cysteine-rich repeats.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
laminin subunit alpha-1
NCBI Official Synonym Full Names
laminin, alpha 1
NCBI Official Symbol
Lama1
NCBI Official Synonym Symbols
Lama; AA408497
NCBI Protein Information
laminin subunit alpha-1
UniProt Protein Name
Laminin subunit alpha-1
Protein Family
UniProt Gene Name
Lama1
UniProt Synonym Gene Names
Lama; Lama-1; S-LAM alpha

Uniprot Description

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Research Articles on LAMA1

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Product Notes

The Mouse LAMA1 lama1 (Catalog #AAA9717807) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717807 ELISA Kit recognizes Mouse LAMA1. It is sometimes possible for the material contained within the vial of "Laminin Subunit alpha-1 (LAMA1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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