Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 293T, 3) HepG2 cells using NFkB p65 Monoclonal Antibody at dilution of 1:2000.)
Mouse NFkB p65 Monoclonal Antibody | anti-NFkB p65 antibody
NFkB p65 Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A Purification
Synonyms
NFkB p65; Monoclonal Antibody; NFkB p65 Monoclonal Antibody; RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; anti-NFkB p65 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
9B4
Purity/Purification
Protein A Purification
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-NFkB p65 antibody
Western Blot (WB), Immunohistochemistry Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:1000 - 1:3000
IHC: 1:100 - 1:300
IP: 1:100 - 1:300
IHC: 1:100 - 1:300
IP: 1:100 - 1:300
Immunogen
Synthetic Peptide
Buffer
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 293T, 3) HepG2 cells using NFkB p65 Monoclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 293T, 3) HepG2 cells using NFkB p65 Monoclonal Antibody at dilution of 1:2000.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Mouse hippocampus tissue using NFkB p65 Monoclonal Antibody at dilution of 1:200.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Mouse hippocampus tissue using NFkB p65 Monoclonal Antibody at dilution of 1:200.)
Related Product Information for anti-NFkB p65 antibody
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel,are members of a family of transcription factors that include the two subunits of the transcription factor NF?B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp ?B sequence in the immunoglobulin ? light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF?B is activated and NF?B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
Product Categories/Family for anti-NFkB p65 antibody
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Calculated MW: 60
Observed MW: 65
Observed MW: 65
Similar Products
Product Notes
The NFkB p65 (Catalog #AAA2534075) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NFkB p65 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NFkB p65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:1000 - 1:3000 IHC: 1:100 - 1:300 IP: 1:100 - 1:300. Researchers should empirically determine the suitability of the NFkB p65 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB p65, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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