Rabbit PDGFRB Polyclonal Antibody | anti-PDGFRB antibody

Anti-PDGFRB Antibody

Cat. Num. AAA4150033

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: PDGFRB; Polyclonal Antibody; Anti-PDGFRB Antibody; anti-PDGFRB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using PDGFRB antibody.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PDGFRB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:20- 1:50
• Immunogen: Recombinant protein of human PDGFRB
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Related Product Information for anti-PDGFRB antibody

Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor +/- (PDGFR+/-) and PDGF receptor 2 (PDGFR2). PDGFR+/- and PDGFR2 share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFR+/- homodimers bind all PDGF isoforms except those containing PDGF D. PDGFR2 homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor +/-/2 binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFR+/- and PDGFR2 can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLC3, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFR2 is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFR2 (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFR2 (7). Tyr740 is also required for PDGFR2-mediated PI3 kinase activation (8).