Rabbit PDGFD Polyclonal Antibody | anti-PDGFD antibody

PDGFD Antibody

Cat. Num. AAA9611442

• Gene Names: PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PDGFD; Polyclonal Antibody; PDGFD Antibody; IEGF; Iris expressed growth factor; Iris-expressed growth factor; MGC26867; MSTP036; PDGF D; PDGF-D; PDGFD latent form; PDGFD receptor-binding form; PDGFD_HUMAN; Platelet derived growth factor D; Platelet-derived growth factor D; receptor-binding form; rSCDGF-B; SCDGF B; SCDGF-B; SCDGFB; Spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal-cord derived growth factor-B protein; anti-PDGFD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PDGFD Antibody detects endogenous levels of PDGFD.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 364

• Applicable Applications for anti-PDGFD antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human PDGFD.
• Tissue Specificity: Expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen and colon. In the kidney, expressed by the visceral epithelial cells of the glomeruli. A widespread expression is also seen in the medial smooth muscle cells of arteries and arterioles, as well as in smooth muscle cells of vasa rectae in the medullary area. Expressed in the adventitial connective tissue surrounding the suprarenal artery. In chronic obstructive nephropathy, a persistent expression is seen in glomerular visceral epithelial cells and vascular smooth muscle cells, as well as de novo expression by periglomerular interstitial cells and by some neointimal cells of atherosclerotic vessels. Expression in normal prostate is seen preferentially in the mesenchyme of the gland while expression is increased and more profuse in prostate carcinoma. Expressed in many ovarian, lung, renal and brain cancer-derived cell lines.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Extracellular Region or Secreted
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Rat brain, using PDGFD Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Human prostate cancer tissue, using PDGFD Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-PDGFD antibody

Description: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix (By similarity).

Post Translational Modifications: Activated by proteolytic cleavage. Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after Arg-247 or Arg-249 by urokinase plasminogen activator gives rise to the active form.

Subunit Structure: Homodimer; disulfide-linked. Interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB.

Similarity: Belongs to the PDGF/VEGF growth factor family.

NCBI and Uniprot Product Information

• NCBI GI #: 15451921
• NCBI GeneID: 80310
• NCBI Accession #: NP_149126.1
• NCBI GenBank Nucleotide #: NP_149126.1
• UniProt Accession #: Q9GZP0
• Molecular Weight: Observed: 43 kDa; Predicted: 43 kDa
• NCBI Official Full Name: platelet-derived growth factor D isoform 2
• NCBI Official Synonym Full Names: platelet derived growth factor D
• NCBI Official Symbol: PDGFD
• NCBI Official Synonym Symbols: IEGF; SCDGFB; MSTP036; SCDGF-B
• NCBI Protein Information: platelet-derived growth factor D
• UniProt Protein Name: Platelet-derived growth factor D
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFD
• UniProt Synonym Gene Names: IEGF; SCDGFB; PDGF-D; SCDGF-B; PDGFD latent form
• UniProt Entry Name: PDGFD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding

Biological Process: multicellular organismal development; positive regulation of cell division; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation