Rabbit PDGF Polyclonal Antibody | anti-PDGFA antibody

RABBIT ANTI HUMAN PDGF AA HOMODIMER

Cat. Num. AAA221730

• Gene Names: PDGFA; PDGF1; PDGF-A
• Applications: ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: PDGF; Polyclonal Antibody; RABBIT ANTI HUMAN PDGF AA HOMODIMER; anti-PDGFA antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 211

• Applicable Applications for anti-PDGFA antibody: ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: Maximum Dilution: 0.5ug/ml;; Functional Assays: Minimum Dilution: 0.17; Maximum Dilution: 0.19ug/ml;; Immunohistology - Paraffin: Application Note: This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose. ; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
• Immunogen: Recombinant human PDGF-AA.; Buffer Solution
• Antiserum Preparation: Antisera to human PDGF-AA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded human skin stained with Rabbit anti Human PDGF-AA (MBS221730) following enzyme mediated antigen retrieval using proteinase K)

Testing Data #2

(Formalin fixed, paraffin embedded human skin stained with Rabbit anti Human PDGF-AA (MBS221730) following enzyme mediated antigen retrieval using proteinase K)

Testing Data #3

(Formalin fixed, paraffin embedded human skin stained with Rabbit anti Human PDGF-AA (MBS221730) following enzyme mediated antigen retrieval using proteinase K)

Testing Data #4

(Formalin fixed, paraffin embedded human skin stained with Rabbit anti Human PDGF-AA (MBS221730) following enzyme mediated antigen retrieval using proteinase K)

Related Product Information for anti-PDGFA antibody

Rabbit anti Human PDGF AA Homodimer antibody recognizes human PDGF-AA (Platelet derived growth factor A chain), a disulphide-linked alpha-chain homodimer and member of the PDGF family, released from platelet alpha-granules following trauma/platelet activation. Naturally occurring active forms of PDGF exist as dimers of two alpha chains (AA), two beta chains (BB), or a combination of both chains (AB), all of which signal through the high-affinity PDGF-Ralpha receptor, and the latter two of which can also signal through the PDGF-Rbeta receptor. PDGFs are potent mitogens for a variety of cells including those of the connective tissue, bone and cartilage, blood and smooth muscle cells, and are involved in several biological processes including chemotaxis, hyperplasia and embryonic neurogenesis. Increased expression of PDGF-AA has been associated with a diverse range of conditions including progression of osteosarcomas, persistent Cytomegalovirus infection in kidney allografts and mediation of IL-13-stimulated lung fibroblast proliferation in asthma.

NCBI and Uniprot Product Information

• NCBI GI #: 77695917
• NCBI GeneID: 5154
• NCBI Accession #: NP_002598.4
• NCBI GenBank Nucleotide #: NM_002607.5
• UniProt Accession #: P04085; B5BU73
• Molecular Weight: 22,253 Da
• NCBI Official Full Name: platelet-derived growth factor subunit A isoform 1 preproprotein
• NCBI Official Synonym Full Names: platelet-derived growth factor alpha polypeptide
• NCBI Official Symbol: PDGFA
• NCBI Official Synonym Symbols: PDGF1; PDGF-A
• NCBI Protein Information: platelet-derived growth factor subunit A; PDGF A-chain; PDGF subunit A; PDGF-1; platelet-derived growth factor A chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein
• UniProt Protein Name: Platelet-derived growth factor subunit A
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFA
• UniProt Synonym Gene Names: PDGF1; PDGF subunit A
• UniProt Entry Name: PDGFA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Cell cycle regulation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: Golgi membrane; extracellular space; cell surface; microvillus; endoplasmic reticulum lumen; extracellular region

Molecular Function: collagen binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding

Biological Process: skin development; extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; response to estradiol stimulus; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; regulation of smooth muscle cell migration; platelet degranulation; cell-cell signaling; hair follicle development; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; inner ear development; epidermal growth factor receptor signaling pathway; response to drug; platelet activation; response to retinoic acid; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; response to inorganic substance; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of protein kinase B signaling cascade; organ morphogenesis; positive regulation of cell division; regulation of actin cytoskeleton organization and biogenesis; response to hypoxia; innate immune response; actin cytoskeleton organization and biogenesis; blood coagulation; alveolus development; positive regulation of DNA replication; positive regulation of cell migration