Mouse anti-Human PDE6D Monoclonal Antibody | anti-PDE6D antibody

PDE6D (Retinal Rod Rhodopsin-sensitive cGMP 3',5'-cyclic Phosphodiesterase Subunit delta, GMP-PDE delta, Protein p17, PDED)

Cat. Num. AAA6012888

• Gene Names: PDE6D; PDED
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PDE6D; Monoclonal Antibody; PDE6D (Retinal Rod Rhodopsin-sensitive cGMP 3'; 5'-cyclic Phosphodiesterase Subunit delta; GMP-PDE delta; Protein p17; PDED); Anti -PDE6D (Retinal Rod Rhodopsin-sensitive cGMP 3'; anti-PDE6D antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 5C7
• Specificity: Recognizes human PDE6D.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV

• Applicable Applications for anti-PDE6D antibody: ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA and Western Blot.; Dilution: Immunofluorescence: 20ug/ml
• Immunogen: Full length recombinant corresponding to aa1-151 from human PDE6D (AAH07831) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (42.24kD).)

Western Blot (WB)

(PDE6D monoclonal antibody. Western Blot analysis of PDE6D expression in HeLa.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PDE6D on HeLa cell. [antibody concentration 20ug/ml])

Testing Data

(Detection limit for recombinant GST tagged PDE6D is ~0.03ng/ml as a capture antibody.)

Related Product Information for anti-PDE6D antibody

Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation.

Product Categories/Family for anti-PDE6D antibody

Antibodies; Abs to Enzymes; Phosphodiesterase

NCBI and Uniprot Product Information

• NCBI GI #: 48146077
• NCBI GeneID: 5147
• UniProt Accession #: Q6IB24; O43924
• Molecular Weight: 17,420 Da
• NCBI Official Full Name: PDE6D
• NCBI Official Synonym Full Names: phosphodiesterase 6D, cGMP-specific, rod, delta
• NCBI Official Symbol: PDE6D
• NCBI Official Synonym Symbols: PDED
• NCBI Protein Information: retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta; protein p17; GMP-PDE delta
• UniProt Protein Name: PDE6D protein
• Protein Family: Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE6D
• UniProt Synonym Gene Names: tmp_locus_12
• UniProt Entry Name: Q6IB24_HUMAN

NCBI Description

This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]

Uniprot Description

PDE6D: Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. Belongs to the PDE6D/unc-119 family.

Chromosomal Location of Human Ortholog: 2q35-q36

Cellular Component: cytoplasmic vesicle membrane; cytoskeleton; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; 3',5'-cyclic-nucleotide phosphodiesterase activity; GTPase inhibitor activity; Rab GTPase binding

Biological Process: visual perception; metabolic process; organelle organization and biogenesis; response to stimulus; negative regulation of catalytic activity

Disease: Joubert Syndrome 22