Rabbit anti-Mouse PDE1B Polyclonal Antibody | anti-PDE1B antibody

PDE1B, CT (PDE1B, PDE1B1, PDES1B, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, 63kD Cam-PDE) (MaxLight 650)

Cat. Num. AAA6332097

• Gene Names: PDE1B; PDE1B1; PDES1B; HEL-S-79p
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDE1B; Polyclonal Antibody; CT (PDE1B; PDE1B1; PDES1B; Calcium/calmodulin-dependent 3'; 5'-cyclic nucleotide phosphodiesterase 1B; 63kD Cam-PDE) (MaxLight 650); 63kD Cam-PDE; anti-PDE1B antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-PDE1B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PDE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-508 amino acids from the C-terminal region of human PDE1B.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-PDE1B antibody

Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).

Product Categories/Family for anti-PDE1B antibody

Antibodies; Calmodulin

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Vandeput, F., et al. J. Biol. Chem. 282(45):32749-32757(2007). Bender, A.T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(2):460-465(2006). Bender, A.T., et al. Proc. Natl. Acad. Sci. U.S.A. 102(2):497-502(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4505677
• NCBI GeneID: 5153
• NCBI Accession #: NP_000915.1; NP_001159447.1
• NCBI GenBank Nucleotide #: NM_000924.3
• UniProt Accession #: Q01064; Q92825; Q96KP3
• Molecular Weight: 59,051 Da
• NCBI Official Full Name: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 1B
• NCBI Official Symbol: PDE1B
• NCBI Official Synonym Symbols: PDE1B1; PDES1B; HEL-S-79p
• NCBI Protein Information: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
• UniProt Protein Name: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
• UniProt Gene Name: PDE1B
• UniProt Synonym Gene Names: PDE1B1; PDES1B; Cam-PDE 1B
• UniProt Entry Name: PDE1B_HUMAN

NCBI Description

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

PDE1B: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase; Apoptosis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell soma; cytosol

Molecular Function: calmodulin binding; 3',5'-cyclic-AMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; monocyte differentiation; apoptosis; regulation of neurotransmitter levels; response to amphetamine; cAMP catabolic process; regulation of dopamine metabolic process; locomotory behavior; signal transduction; phospholipase C activation; innate immune response; visual learning; cGMP catabolic process; blood coagulation; serotonin metabolic process