Rabbit anti-Mouse PDE1B Polyclonal Antibody | anti-PDE1B antibody

PDE1B, CT (PDE1B, PDE1B1, PDES1B, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, 63kD Cam-PDE) (Biotin)

Cat. Num. AAA6332091

• Gene Names: PDE1B; PDE1B1; PDES1B; HEL-S-79p
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDE1B; Polyclonal Antibody; CT (PDE1B; PDE1B1; PDES1B; Calcium/calmodulin-dependent 3'; 5'-cyclic nucleotide phosphodiesterase 1B; 63kD Cam-PDE) (Biotin); 63kD Cam-PDE; anti-PDE1B antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PDE1B antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PDE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-508 amino acids from the C-terminal region of human PDE1B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PDE1B Antibody (C-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the PDE1B antibody detected the PDE1B protein (arrow).)

Immunohistochemistry (IHC)

(PDE1B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PDE1B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PDE1B antibody

Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).

Product Categories/Family for anti-PDE1B antibody

Antibodies; Calmodulin

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Vandeput, F., et al. J. Biol. Chem. 282(45):32749-32757(2007). Bender, A.T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(2):460-465(2006). Bender, A.T., et al. Proc. Natl. Acad. Sci. U.S.A. 102(2):497-502(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4505677
• NCBI GeneID: 5153
• NCBI Accession #: NP_000915.1; NP_001159447.1
• NCBI GenBank Nucleotide #: NM_000924.3
• UniProt Accession #: Q01064; Q92825; Q96KP3
• Molecular Weight: 59,051 Da
• NCBI Official Full Name: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 1B
• NCBI Official Symbol: PDE1B
• NCBI Official Synonym Symbols: PDE1B1; PDES1B; HEL-S-79p
• NCBI Protein Information: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
• UniProt Protein Name: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
• UniProt Gene Name: PDE1B
• UniProt Synonym Gene Names: PDE1B1; PDES1B; Cam-PDE 1B
• UniProt Entry Name: PDE1B_HUMAN

NCBI Description

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

PDE1B: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase; Apoptosis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell soma; cytosol

Molecular Function: calmodulin binding; 3',5'-cyclic-AMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; monocyte differentiation; apoptosis; regulation of neurotransmitter levels; response to amphetamine; cAMP catabolic process; regulation of dopamine metabolic process; locomotory behavior; signal transduction; phospholipase C activation; innate immune response; visual learning; cGMP catabolic process; blood coagulation; serotonin metabolic process