Western Blot (WB)
(Western blot analysis of HAS3 expression in A549 (A), SKOV3 (B), mouse heart (C), rat liver (D) whole cell lysates.)
Rabbit anti-Human HAS3 Polyclonal Antibody | anti-HAS3 antibody
Anti-HAS3 Antibody
Western Blot (WB)
(Western blot analysis of HAS3 expression in A549 (A), SKOV3 (B), mouse heart (C), rat liver (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of HAS3 expression in A549 (A), SKOV3 (B), mouse heart (C), rat liver (D) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of HAS3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of HAS3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. This gene is a member of the NODC/HAS gene family. Compared to the proteins encoded by other members of this gene family, this protein appears to be more of a regulator of hyaluronan synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
Uniprot Description
HAS3: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. Belongs to the NodC/HAS family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Transferase; Membrane protein, multi-pass; EC 2.4.1.212
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: cytoplasm; integral to membrane; integral to plasma membrane; plasma membrane
Molecular Function: hyaluronan synthase activity
Biological Process: carbohydrate metabolic process; cell adhesion; extracellular polysaccharide biosynthetic process; hyaluronan biosynthetic process; positive regulation of transcription, DNA-dependent
Research Articles on HAS3
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Product Notes
The HAS3 has3 (Catalog #AAA8245839) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HAS3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HAS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/10 - 1/100. Researchers should empirically determine the suitability of the HAS3 has3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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