Immunohistochemistry (IHC)
(Immunochemical staining of human PDE1A in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human PDE1A Polyclonal Antibody | anti-PDE1A antibody
Anti-PDE1A Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
PDE1A; Polyclonal Antibody; Anti-PDE1A Antibody; Rabbit Polyclonal; PDE1A Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CAM-PDE-1A Antibody; Anti-HCAM-1 Antibody; Anti-HCAM1 Antibody; Anti-HSPDE1A Antibody; anti-PDE1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PDE1A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PDE1A antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human PDE1A fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PDE1A fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human PDE1A in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PDE1A in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-PDE1A antibody
A reduction in intracellular calcium causes an increase in intracellular cAMP and PKA activity through stimulation of calcium inhibitable adenylyl cyclase 6 and inhibition of phosphodiesterase 1 (PDE1), the main enzymes generating and degrading cAMP in the distal nephron and collecting duct. PDE1, a subfamily of cyclic nucleotide PDEs consisting of three isoforms, PDE1A, PDE1B and PDE1C, regulates vascular tone. The PDE1A gene spans over 120 kb of genomic DNA, and consists of at least 17 exons and 16 introns. PDE1A is the dominant PDE1 isoform present in vascular smooth muscle cells (VSMCs), and relaxation mediated by PDE1A inhibition is predominantly driven by enhanced cGMP signalling.
References
Wang X, et al. (2017) Generation and phenotypic characterization of pde1a mutant mice. PLoS One 12 (7): e0181087.Michibata H, et al. (2001) Human ca2+/calmodulin-dependent phosphodiesterase pde1a: Novel splice variants, their specific expression, genomic organization, and chromosomal localization. Biochim Biophys Acta 1517 (2): 278-287.Khammy MM, et al. (2017) Pde1a inhibition elicits cgmp-dependent relaxation of rat mesenteric arteries. Br J Pharmacol 174 (22): 4186-4198.
Similar Products
Product Notes
The PDE1A (Catalog #AAA8110191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDE1A Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDE1A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the PDE1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
