Rabbit anti-Human Plastin 3 (PLS3) Polyclonal Antibody | anti-PLS3 antibody

Polyclonal Antibody to Plastin 3 (PLS3)

Cat. Num. AAA2004363

• Gene Names: PLS3; BMND18; T-plastin
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Plastin 3 (PLS3); Polyclonal Antibody; Polyclonal Antibody to Plastin 3 (PLS3); anti-PLS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against PLS3. It has been selected for its ability to recognize PLS3 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 0.57mg/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF- AL TKPENQDIDW TLLEGETREE RTFRNWMNSL GVNPHVNHLY ADLQDALVIL QLYERIKVPV DWSKVNKPPY PKLGANMKKL ENCNYAVELG KHPAKFSLVG IGGQDLNDGN QTLTLALVWQ LMRRYTLNVL EDLGDGQKAN DDIIVNWVNR TLSEAGKSTS IQSFKDKTIS SSLAVVDLID AIQPGCINYD LVKSGNLTED DKHNNAKYAV SMARRIGARV YALPEDLVEV KPKMVMTVFA CLMGRGMKRV
• Sequence Length: 617

• Applicable Applications for anti-PLS3 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant PLS3 (Ala379~Val630) expressed in E.coli.
• Cross Reactivity: Human, Mouse, Pig
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2079718
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Heart Tissue; Lane3: Mouse Liver Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant PLS3, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 209862851
• NCBI GeneID: 5358
• NCBI Accession #: NP_001129497.1
• NCBI GenBank Nucleotide #: NM_001136025.4
• UniProt Accession #: P13797; Q86YI6; A8K579; B1AQ09; B4DGB4; B7Z6M1
• Molecular Weight: 65,632 Da
• NCBI Official Full Name: plastin-3 isoform 1
• NCBI Official Synonym Full Names: plastin 3
• NCBI Official Symbol: PLS3
• NCBI Official Synonym Symbols: BMND18; T-plastin
• NCBI Protein Information: plastin-3
• UniProt Protein Name: Plastin-3
• Protein Family: Plastin
• UniProt Gene Name: PLS3

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

PLS3: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: actin filament; actin filament bundle; cytoplasm; cytosol; plasma membrane

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin crosslink formation; actin filament bundle formation; actin filament network formation

Disease: Bone Mineral Density Quantitative Trait Locus 18