Western Blot (WB)
(Western blot analysis of PDCD4 expression in HeLa cell lysate.)
Rabbit anti-Human, Mouse PDCD4 Polyclonal Antibody | anti-PDCD4 antibody
Anti-PDCD4 Rabbit pAb
Gene Names
PDCD4; H731
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
PDCD4; Polyclonal Antibody; Anti-PDCD4 Rabbit pAb; Death up-regulated gene protein; Dug; H731; Ma3; Neoplastic transformation inhibitor; Nuclear antigen H731; Pdcd4; Programmed cell death protein 4; Tis; anti-PDCD4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PDCD4 Antibody detects endogenous levels of total PDCD4
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
469
Applicable Applications for anti-PDCD4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:100-1:500
ICC/IF: 1:50-1:200
FC/FACS: 1:50
IHC: 1:100-1:500
ICC/IF: 1:50-1:200
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human PDCD4
Subcellular Location
Cytoplasm
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of PDCD4 expression in HeLa cell lysate.)
Western Blot (WB)
(Western blot analysis of PDCD4 expression in HeLa cell lysate.)
Related Product Information for anti-PDCD4 antibody
PDCD4 (programmed cell death protein 4) was identified as a suppressor of neoplastic transformation. It interacts with eukaryotic initiation factors eIF4AI and eIF4AII. PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52kDa
NCBI Official Full Name
programmed cell death protein 4 isoform 1
NCBI Official Synonym Full Names
programmed cell death 4
NCBI Official Symbol
PDCD4
NCBI Official Synonym Symbols
H731
NCBI Protein Information
programmed cell death protein 4
UniProt Protein Name
Programmed cell death protein 4
Protein Family
UniProt Gene Name
PDCD4
UniProt Synonym Gene Names
H731
NCBI Description
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
Uniprot Description
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA ().
Research Articles on PDCD4
Similar Products
Product Notes
The PDCD4 pdcd4 (Catalog #AAA4751041) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDCD4 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDCD4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:100-1:500 ICC/IF: 1:50-1:200 FC/FACS: 1:50. Researchers should empirically determine the suitability of the PDCD4 pdcd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDCD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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