Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCYT1A) Polyclonal Antibody, Unconjugated (bs-11306R) at 1:400 overnight at 4°C)
Rabbit PCYT1A Polyclonal Antibody | anti-PCYT1A antibody
PCYT1A Polyclonal Antibody
Gene Names
PCYT1A; CT; CTA; CCTA; CTPCT; PCYT1
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep
Applications
Immunohistochemistry
Purity
affinity purified by Protein A
Synonyms
PCYT1A; Polyclonal Antibody; PCYT1A Polyclonal Antibody; CCT A; CCT alpha; CCT-alpha; Choline phosphate cytidylyltransferase A; Choline-phosphate cytidylyltransferase A; CT A; CT antibody CTP:phosphocholine cytidylyltransferase A; CTPCT; PCY1A_HUMAN; PCYT1; Pcyt1a; Phosphate cytidylyltransferase 1 choline alpha; Phosphate cytidylyltransferase 1 choline alpha isoform; Phosphorylcholine transferase A; anti-PCYT1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep
Clonality
Polyclonal
Specificity
PCYT1A Polyclonal Antibody detects endogenous levels of PCYT1A protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-PCYT1A antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-500
Immunogen
KLH conjugated synthetic peptide derived from human PCYT1A
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCYT1A) Polyclonal Antibody, Unconjugated (bs-11306R) at 1:400 overnight at 4°C)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCYT1A) Polyclonal Antibody, Unconjugated (bs-11306R) at 1:400 overnight at 4°C)
Related Product Information for anti-PCYT1A antibody
Increase in fetal surfactant synthesis and lung maturity is caused by the glucocorticoidal induction of enzymes required for phosphatidylcholine synthesis towards the end of gestation (1). The regulation of gestational age-dependent induction of phosphatidylcholine synthesis by glucocorticoids is still unclear (1). The rate-controlling enzyme in the phosphatidylcholine biosynthetic pathway is CTP-phosphocholine cytidylyltransferase A (CCT A) (2-4). In cultured eukaryotic cells, this enzyme is essential for survival (3). The alpha isoform is located in the nucleus and is regulated by reversible phosphorylation and membrane association (3). There is significant identity between the alpha-helical membrane-binding domains of CCT A and soybean oleosin (2). Expressed CCT A has lipid-dependent cytidylyltransferase activity (5). The gene which encodes CCT A maps to human chromosome 3q (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,731 Da
NCBI Official Full Name
CTP:phosphocholine cytidylyltransferase
NCBI Official Synonym Full Names
phosphate cytidylyltransferase 1, choline, alpha
NCBI Official Symbol
PCYT1A
NCBI Official Synonym Symbols
CT; CTA; CCTA; CTPCT; PCYT1
NCBI Protein Information
choline-phosphate cytidylyltransferase A; CT A; CCT A; CCT-alpha; phosphorylcholine transferase A; CTP:phosphocholine cytidylyltransferase A
UniProt Protein Name
Choline-phosphate cytidylyltransferase A
Protein Family
UniProt Gene Name
PCYT1A
UniProt Synonym Gene Names
CTPCT; PCYT1; CCT A; CT A
UniProt Entry Name
PCY1A_HUMAN
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Product Notes
The PCYT1A pcyt1a (Catalog #AAA3015215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCYT1A Polyclonal Antibody reacts with Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's PCYT1A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-500. Researchers should empirically determine the suitability of the PCYT1A pcyt1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCYT1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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