Rabbit anti-Human PCTAIRE3 Polyclonal Antibody | anti-PCTAIRE3 antibody

PCTAIRE3, NT (PCTAIRE-motif Protein Kinase 3, PCTAIRE 3, PCTK3, Cell Division Protein Kinase 18, Cyclin-dependent Kinase 18, CDK18, Serine/threonine Protein Kinase PCTAIRE-3) (Azide free) (HRP)

Cat. Num. AAA6492406

• Gene Names: CDK18; PCTK3; PCTAIRE; PCTAIRE3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PCTAIRE3; Polyclonal Antibody; NT (PCTAIRE-motif Protein Kinase 3; PCTAIRE 3; PCTK3; Cell Division Protein Kinase 18; Cyclin-dependent Kinase 18; CDK18; Serine/threonine Protein Kinase PCTAIRE-3) (Azide free) (HRP); EC=2.7.11.22; anti-PCTAIRE3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PCTAIRE3.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PCTAIRE3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 25-55 in the N-terminal region of human PCTAIRE3, UniProt Accession #Q07002.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) using MBS630513 Lane 1: nontransfected Lane 2: transiently transfected with PCTAIRE3.)

Western Blot (WB)

(Western Blot analysis of HL-60 cell lysates to usingMBS630513. This demonstrates that MBS630513 detected the PCTAIRE3 protein.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin-embedded human breast carcinoma tissue using MBS630513 followed by peroxidase-conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS630513 for immunohistochemistry. BC = breast carcinoma.)

Related Product Information for anti-PCTAIRE3 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway The CMGC group consists of 60 kinases including the cyclin-dependent kinase (CDK) and close relatives family, the MAP kinase (ERK) family, the glycogen synthase kinase 3 (GSK3) family, and the Cdc2-like kinase (CLK) family.

Product Categories/Family for anti-PCTAIRE3 antibody

Antibodies; Protein Kinases

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Meyerson, M., et al., EMBO J. 11(8):2909-2917 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 47078229
• NCBI GeneID: 5129
• NCBI Accession #: NP_002587
• NCBI GenBank Nucleotide #: NM_002596
• UniProt Accession #: Q07002; Q5VXQ2; Q6V3A2; Q6V3A3; Q96F90
• Molecular Weight: 57,577 Da
• NCBI Official Full Name: cyclin-dependent kinase 18 isoform b
• NCBI Official Synonym Full Names: cyclin-dependent kinase 18
• NCBI Official Symbol: CDK18
• NCBI Official Synonym Symbols: PCTK3; PCTAIRE; PCTAIRE3
• NCBI Protein Information: cyclin-dependent kinase 18; PCTAIRE protein kinase 3; PCTAIRE-motif protein kinase 3; cell division protein kinase 18; serine/threonine-protein kinase PCTAIRE-3
• UniProt Protein Name: Cyclin-dependent kinase 18
• UniProt Gene Name: CDK18
• UniProt Synonym Gene Names: PCTAIRE3; PCTK3
• UniProt Entry Name: CDK18_HUMAN