Western Blot (WB)
((2ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat anti-Human, Mouse AKT1 Polyclonal Antibody | anti-AKT1 antibody
Goat anti-AKT1 Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
AKT1; Polyclonal Antibody; Goat anti-AKT1 Antibody; v-akt murine thymoma viral oncogene homolog 1; AKT; MGC99656; PKB; PRKBA; RAC; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; murine thymoma viral (v-akt) oncogene homolog-1; protein kinase B; rac protein kinase alpha; anti-AKT1 antibody
Host
Goat
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QDVDQREAPLN
Sequence Length
480
Applicable Applications for anti-AKT1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Western Blot: Approx 80+60kDa bands observed in lysates of cell line HeLa and Jurkat (calculated MW of 55.7kDa according to NP_005154.2). The observed 80kDa band is considered representing the acetylated protein. Recommended concentration: 1-3ug/ml.
Peptide ELISA: antibody detection limit dilution 1:4000.
Peptide ELISA: antibody detection limit dilution 1:4000.
Epitope
Internal Region
Immunogen
Peptide with sequence C-QDVDQREAPLN, from the internal region of the protein sequence according to NP_005154.2; NP_001014431.1; NP_001014432.1.
Corresponding Immunizing Peptide
Important Note
All variants represent identical protein (NP_005154.2, NP_001014431.1, NP_001014432.1). This antibody is NOT expected to cross-react with AKT2. It may recognize AKT1 in Rat and Dog.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((2ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,347 Da
NCBI Official Full Name
RAC-alpha serine/threonine-protein kinase
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 1
NCBI Official Symbol
AKT1
NCBI Official Synonym Symbols
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
NCBI Protein Information
RAC-alpha serine/threonine-protein kinase; AKT1m; PKB alpha; RAC-PK-alpha; proto-oncogene c-Akt; protein kinase B alpha; rac protein kinase alpha
UniProt Protein Name
RAC-alpha serine/threonine-protein kinase
Protein Family
UniProt Gene Name
AKT1
UniProt Synonym Gene Names
PKB; RAC; PKB; PKB alpha
UniProt Entry Name
AKT1_HUMAN
Similar Products
Product Notes
The AKT1 akt1 (Catalog #AAA422624) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-AKT1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AKT1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Western Blot: Approx 80+60kDa bands observed in lysates of cell line HeLa and Jurkat (calculated MW of 55.7kDa according to NP_005154.2). The observed 80kDa band is considered representing the acetylated protein. Recommended concentration: 1-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:4000. Researchers should empirically determine the suitability of the AKT1 akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QDVDQREAPL N. It is sometimes possible for the material contained within the vial of "AKT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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