Western Blot (WB)
(PCOTH Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PCOTH antibody detected the PCOTH protein (arrow).)
Rabbit anti-Human PCOTH Polyclonal Antibody | anti-PCOTH antibody
PCOTH, ID (PCOTH, Protein PCOTH, Prostate collagen triple helix protein) (APC)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PCOTH; Polyclonal Antibody; ID (PCOTH; Protein PCOTH; Prostate collagen triple helix protein) (APC); Prostate collagen triple helix protein; anti-PCOTH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PCOTH antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PCOTH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the Central region of human PCOTH.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PCOTH Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PCOTH antibody detected the PCOTH protein (arrow).)
Western Blot (WB)
(PCOTH Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PCOTH antibody detected the PCOTH protein (arrow).)
Immunohistochemistry (IHC)
(PCOTH antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCOTH antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PCOTH antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCOTH antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PCOTH Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PCOTH Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PCOTH antibody
May be involved in growth and survival of prostate cancer cells through the TAF-Ibeta pathway.
Product Categories/Family for anti-PCOTH antibody
References
Anazawa, Y., et al. Cancer Res. 65(11):4578-4586(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prostate collagen triple helix protein isoform a
UniProt Protein Name
Protein PCOTH
UniProt Gene Name
PCOTH
UniProt Entry Name
PCOTH_HUMAN
Uniprot Description
Function: May be involved in growth and survival of prostate cancer cells through the TAF-Ibeta pathway. Ref.2
Subcellular location: Cytoplasm Ref.2.
Tissue specificity: Expressed in prostate and testis. Weakly or not expressed in other tissues. Overexpressed in prostate cancers. Ref.2
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Product Notes
The PCOTH pcoth (Catalog #AAA6331837) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCOTH, ID (PCOTH, Protein PCOTH, Prostate collagen triple helix protein) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCOTH can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PCOTH pcoth for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCOTH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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