Rabbit PCNA Polyclonal Antibody | anti-PCNA antibody

Anti-PCNA Antibody

Cat. Num. AAA419418

• Gene Names: PCNA; ATLD2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: PCNA; Polyclonal Antibody; Anti-PCNA Antibody; anti-PCNA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using PCNA antibody. Immunohistochemistry of paraffin-embedded Breast cancer using PCNA antibody. Immunohistochemistry of paraffin-embedded human thyroid cancer using PCNA antibody at dilution of 1:200 (200x lens). Immunohistochemistry of paraffin-embedded rat brain using PCNA antibody at dilution of 1:200 (200x lens). Immunofluorescence analysis of A549 cell using PCNA antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-PCNA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:20-1:50; IP: 1:20-1:50
• Immunogen: Recombinant protein of human PCNA
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Related Product Information for anti-PCNA antibody

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.

NCBI and Uniprot Product Information

• NCBI GI #: 49456555
• NCBI GeneID: 5111
• Molecular Weight: 28,769 Da
• NCBI Official Full Name: PCNA, partial
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Official Synonym Symbols: ATLD2
• NCBI Protein Information: proliferating cell nuclear antigen
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: PCNA-associated factor
• UniProt Gene Name: PCNA
• UniProt Synonym Gene Names: PCNA

NCBI Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Uniprot Description

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: Cell cycle regulation; DNA replication

Chromosomal Location of Human Ortholog: 20p12.3

Cellular Component: centrosome; cytoplasm; DNA replication factor C complex; nuclear chromosome, telomeric region; nuclear replication fork; nucleoplasm; nucleus; PCNA complex; replication fork

Molecular Function: chromatin binding; damaged DNA binding; dinucleotide insertion or deletion binding; DNA polymerase processivity factor activity; enzyme binding; histone acetyltransferase binding; identical protein binding; MutLalpha complex binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity; receptor tyrosine kinase binding

Biological Process: bypass DNA synthesis; cell proliferation; DNA damage response, detection of DNA damage; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA strand elongation during DNA replication; epithelial cell differentiation; error-prone postreplication DNA repair; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; leading strand elongation; mismatch repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; positive regulation of deoxyribonuclease activity; positive regulation of DNA repair; positive regulation of DNA replication; protein sumoylation; replication fork processing; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair

Disease: Ataxia-telangiectasia-like Disorder 2