Rabbit anti-Human PCNA Polyclonal Antibody | anti-PCNA antibody

RABBIT anti PCNA

Cat. Num. AAA462104

• Gene Names: PCNA; MGC8367
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The Rabbit polyclonal antibody is purified by Affinity Purification
• Synonyms: PCNA; Polyclonal Antibody; RABBIT anti PCNA; Rabbit anti-PCNA; anti-PCNA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. This antibody recognizes human PCNA. The other species are not tested.
• Purity/Purification: The Rabbit polyclonal antibody is purified by Affinity Purification
• Form/Format: This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-PCNA antibody: Western Blot, Immunohistochemistry - Paraffin
• Application Notes: Western Blot: 0.1-1 ug/ml; Immunohistochemistry: 2-5 ug/ml
• Antigen Preparation: Recombinant human PCNA protein
• Positive Control: Tonsil, lymph node, or small intestine
• Cellular Location: Nuclear
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 129694
• NCBI GeneID: 5111
• NCBI Accession #: P12004.1
• NCBI GenBank Nucleotide #: P12004
• UniProt Accession #: P12004; B2R897; D3DW02; Q6FHF5; Q6FI35; Q7Z6A0; Q7Z6A1; Q7Z6A2; Q7Z6A3
• Molecular Weight: 29 kDa
• NCBI Official Full Name: Proliferating cell nuclear antigen
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Official Synonym Symbols: MGC8367
• NCBI Protein Information: proliferating cell nuclear antigen; cyclin; OTTHUMP00000030189; OTTHUMP00000030190; DNA polymerase delta auxiliary protein
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: PCNA-associated factor
• UniProt Gene Name: PCNA
• UniProt Entry Name: PCNA_HUMAN

NCBI Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]

Uniprot Description

Function: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Ref.28 Ref.30

Subunit structure: Homotrimer

By similarity. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A

By similarity. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31

Subcellular location: Nucleus. Note: Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

Post-translational modification: Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.

Miscellaneous: Antibodies against PCNA are present in sera from patients with systemic lupus erythematosus.

Sequence similarities: Belongs to the PCNA family.