Western Blot (WB)
(PCIA1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PCIA1 antibody detected the PCIA1 protein (arrow).)
Rabbit PCIA1 Polyclonal Antibody | anti-DDA1 antibody
PCIA1 Antibody (Center)
Gene Names
DDA1; PCIA1; C19orf58
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Zebrafish, Mouse, Xenopus)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PCIA1; Polyclonal Antibody; PCIA1 Antibody (Center); DET1- and DDB1-associated protein 1; Placenta cross-immune reaction antigen 1; PCIA-1; DDA1; C19orf58; anti-DDA1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Zebrafish, Mouse, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PCIA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the Central region of human PCIA1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
11-40
Sequence Length
102
Applicable Applications for anti-DDA1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:300
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(PCIA1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PCIA1 antibody detected the PCIA1 protein (arrow).)
Western Blot (WB)
(PCIA1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PCIA1 antibody detected the PCIA1 protein (arrow).)
Immunohistochemistry (IHC)
(PCIA1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCIA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PCIA1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCIA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of extracts from 293T cells that were transfected with si-control or transfected si-PCIA1, using rabbit polyclonal PCIA1 Antibody (Center).)
Western Blot (WB)
(Western blot analysis of extracts from 293T cells that were transfected with si-control or transfected si-PCIA1, using rabbit polyclonal PCIA1 Antibody (Center).)
Related Product Information for anti-DDA1 antibody
PCIA1 may be involved in ubiquitination and subsequent proteasomal degradation of target proteins. Component of the DDD-E2 complexes which may provide a platform for interaction with CUL4A and WD repeat proteins.
Product Categories/Family for anti-DDA1 antibody
References
Olma, M.H., et al. J. Cell. Sci. 122 (PT 7), 1035-1044 (2009) :
Pick, E., et al. Mol. Cell. Biol. 27(13):4708-4719(2007)
Li, Z.M., et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 24(3):279-283(2007)
Pick, E., et al. Mol. Cell. Biol. 27(13):4708-4719(2007)
Li, Z.M., et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 24(3):279-283(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11835
NCBI Official Full Name
DET1- and DDB1-associated protein 1
NCBI Official Synonym Full Names
DET1 and DDB1 associated 1
NCBI Official Symbol
DDA1
NCBI Official Synonym Symbols
PCIA1; C19orf58
NCBI Protein Information
DET1- and DDB1-associated protein 1
UniProt Protein Name
DET1- and DDB1-associated protein 1
Protein Family
UniProt Gene Name
DDA1
UniProt Synonym Gene Names
C19orf58; PCIA1; PCIA-1
UniProt Entry Name
DDA1_HUMAN
Uniprot Description
DDA1: May be involved in ubiquitination and subsequent proteasomal degradation of target proteins. Component of the DDD- E2 complexes which may provide a platform for interaction with CUL4A and WD repeat proteins. Belongs to the DDA1 family.
Protein type: Autophagy; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 19p13.11
Research Articles on DDA1
Similar Products
Product Notes
The DDA1 dda1 (Catalog #AAA9204688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 11-40. The PCIA1 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Chicken, Zebrafish, Mouse, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's PCIA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:300. Researchers should empirically determine the suitability of the DDA1 dda1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCIA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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