Rabbit anti-Human PCDHGC3 Polyclonal Antibody | anti-PCDHGC3 antibody

PCDHGC3, ID (PCDHGC3, PCDH2, Protocadherin gamma-C3, Protocadherin-2, Protocadherin-43)

Cat. Num. AAA6005246

• Gene Names: PCDHGC3; PC43; PCDH2; PCDH-GAMMA-C3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PCDHGC3; Polyclonal Antibody; ID (PCDHGC3; PCDH2; Protocadherin gamma-C3; Protocadherin-2; Protocadherin-43); Anti -PCDHGC3; anti-PCDHGC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PCDHGC3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: PCDHGC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 518-547 amino acids from the Central region of human PCDHGC3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PCDHGC3 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PCDHGC3 antibody detected the PCDHGC3 protein (arrow).)

Immunohistochemistry (IHC)

(PCDHGC3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCDHGC3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PCDHGC3 Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PCDHGC3 antibody

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Product Categories/Family for anti-PCDHGC3 antibody

Antibodies; Abs to Cadherin

NCBI and Uniprot Product Information

• NCBI GI #: 14277679
• NCBI GeneID: 5098
• NCBI Accession #: NP_115779.1
• NCBI GenBank Nucleotide #: NM_032403.2
• UniProt Accession #: Q9UN70; O60622; Q08192; Q9Y5C4
• Molecular Weight: 101,077 Da
• NCBI Official Full Name: protocadherin gamma-C3 isoform 3
• NCBI Official Synonym Full Names: protocadherin gamma subfamily C, 3
• NCBI Official Symbol: PCDHGC3
• NCBI Official Synonym Symbols: PC43; PCDH2; PCDH-GAMMA-C3
• NCBI Protein Information: protocadherin gamma-C3; cadherin-like 2; protocadherin 2; protocadherin-2; protocadherin 43; protocadherin-43
• UniProt Protein Name: Protocadherin gamma-C3
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHGC3
• UniProt Synonym Gene Names: PCDH2; PCDH-gamma-C3; PC-43
• UniProt Entry Name: PCDGK_HUMAN

NCBI Description

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]

Uniprot Description

PCDHGC3: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: calcium ion binding

Biological Process: calcium-dependent cell-cell adhesion; homophilic cell adhesion