Rabbit anti-Human PCDHGA5 Polyclonal Antibody | anti-PCDHGA5 antibody

PCDHGA5 Antibody (N-term)

Cat. Num. AAA9209536

• Gene Names: PCDHGA5; ME3; CDH-GAMMA-A5; PCDH-GAMMA-A5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PCDHGA5; Polyclonal Antibody; PCDHGA5 Antibody (N-term); Protocadherin gamma-A5; PCDH-gamma-A5; anti-PCDHGA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PCDHGA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-280 amino acids from the N-terminal region of human PCDHGA5.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 254-280
• Sequence Length: 813

• Applicable Applications for anti-PCDHGA5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(PCDHGA5 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PCDHGA5 Antibody detected the PCDHGA5 protein (arrow).)

Related Product Information for anti-PCDHGA5 antibody

This gene is a member of the protocadherin gamma gene
cluster, one of three related clusters tandemly linked on
chromosome five. These gene clusters have an immunoglobulin-like
organization, suggesting that a novel mechanism may be involved in
their regulation and expression. The gamma gene cluster includes 22
genes divided into 3 subfamilies. Subfamily A contains 12 genes,
subfamily B contains 7 genes and 2 pseudogenes, and the more
distantly related subfamily C contains 3 genes. The tandem array of
22 large, variable region exons are followed by a constant region,
containing 3 exons shared by all genes in the cluster. Each
variable region exon encodes the extracellular region, which
includes 6 cadherin ectodomains and a transmembrane region. The
constant region exons encode the common cytoplasmic region. These
neural cadherin-like cell adhesion proteins most likely play a
critical role in the establishment and function of specific
cell-cell connections in the brain. Alternative splicing has been
described for the gamma cluster genes.

Product Categories/Family for anti-PCDHGA5 antibody

Neuroscience; Signal Transduction

References

Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
Wu, Q., et al. Cell 97(6):779-790(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 11056024
• NCBI GeneID: 56110
• NCBI Accession #: NP_061741.1; NP_114443.1
• NCBI GenBank Nucleotide #: NM_018918.2
• UniProt Accession #: Q9Y5G8; Q2M3F5; Q9Y5D2
• Molecular Weight: 100935
• NCBI Official Full Name: protocadherin gamma-A5 isoform 1
• NCBI Official Synonym Full Names: protocadherin gamma subfamily A, 5
• NCBI Official Symbol: PCDHGA5
• NCBI Official Synonym Symbols: ME3; CDH-GAMMA-A5; PCDH-GAMMA-A5
• NCBI Protein Information: protocadherin gamma-A5
• UniProt Protein Name: Protocadherin gamma-A5
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHGA5
• UniProt Synonym Gene Names: PCDH-gamma-A5
• UniProt Entry Name: PCDG5_HUMAN

NCBI Description

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]

Uniprot Description

PCDHGA5: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: plasma membrane; integral to membrane

Molecular Function: calcium ion binding

Biological Process: homophilic cell adhesion