Rabbit anti-Human PCDHAC2 Polyclonal Antibody | anti-PCDHAC2 antibody

PCDHAC2, ID (PCDHAC2, Protocadherin alpha-C2) (PE)

Cat. Num. AAA6331549

• Gene Names: PCDHAC2; MGC71598; PCDH-ALPHA-C2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PCDHAC2; Polyclonal Antibody; ID (PCDHAC2; Protocadherin alpha-C2) (PE); Protocadherin alpha-C2; anti-PCDHAC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PCDHAC2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PCDHAC2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 616-644 in the central region of human PCDHAC2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6012469. This demonstrates that MBS6012469 detected the PCDHAC2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6012469 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6012469 for immunohistochemistry.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with NCI-H460 cells using MBS6012469 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using MBS6012469 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PCDHAC2 antibody

Antibodies; Cadherin

References

1. Wu, Q., et al. Genome Res. 11(3):389-404(2001). 2. Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000). 3. Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000). 4. Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000). 5. Sugino, H., et al. Genomics 63(1):75-87(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 14165431
• NCBI GeneID: 56134
• NCBI Accession #: NP_114089.1; NP_061722.1
• NCBI GenBank Nucleotide #: NM_031883.2
• UniProt Accession #: Q9Y5I4; Q6P4R2
• Molecular Weight: 109,477 Da
• NCBI Official Full Name: protocadherin alpha-C2 isoform 2
• NCBI Official Synonym Full Names: protocadherin alpha subfamily C, 2
• NCBI Official Symbol: PCDHAC2
• NCBI Official Synonym Symbols: MGC71598; PCDH-ALPHA-C2
• NCBI Protein Information: protocadherin alpha-C2; OTTHUMP00000160007
• UniProt Protein Name: Protocadherin alpha-C2
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHAC2
• UniProt Synonym Gene Names: PCDH-alpha-C2
• UniProt Entry Name: PCDC2_HUMAN

NCBI Description

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq]