Rabbit anti-Human PCDHA7 Polyclonal Antibody | anti-PCDHA7 antibody

PCDHA7, ID (PCDHA7, CNRS4, Protocadherin alpha-7) (PE)

Cat. Num. AAA6331516

• Gene Names: PCDHA7; CNR4; CNRN4; CNRS4; CRNR4; PCDH-ALPHA7
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCDHA7; Polyclonal Antibody; ID (PCDHA7; CNRS4; Protocadherin alpha-7) (PE); Protocadherin alpha-7; anti-PCDHA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PCDHA7 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PCDHA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279-308 amino acids from the Central region of human PCDHA7.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PCDHA7 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PCDHA7 antibody detected the PCDHA7 protein (arrow).)

Immunohistochemistry (IHC)

(PCDHA7 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHA7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PCDHA7 antibody

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Product Categories/Family for anti-PCDHA7 antibody

Antibodies; Cadherin

References

Wu, C., et al. Proteomics 7(11):1775-1785(2007). Wu, Q., et al. Genome Res. 11(3):389-404(2001). Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000). Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000). Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 14165419
• NCBI GeneID: 56141
• NCBI Accession #: NP_114040.1; NP_061733.1
• NCBI GenBank Nucleotide #: NM_031852.1
• UniProt Accession #: Q9UN72; O75282
• Molecular Weight: 100,865 Da
• NCBI Official Full Name: protocadherin alpha-7 isoform 2
• NCBI Official Synonym Full Names: protocadherin alpha 7
• NCBI Official Symbol: PCDHA7
• NCBI Official Synonym Symbols: CNR4; CNRN4; CNRS4; CRNR4; PCDH-ALPHA7
• NCBI Protein Information: protocadherin alpha-7; PCDH-alpha-7; KIAA0345-like 7; OTTHUMP00000223679; ortholog to mouse CNR4
• UniProt Protein Name: Protocadherin alpha-7
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHA7
• UniProt Synonym Gene Names: CNRS4
• UniProt Entry Name: PCDA7_HUMAN

NCBI Description

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq]

Uniprot Description

PCDHA7: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: integral to plasma membrane

Molecular Function: calcium ion binding

Biological Process: nervous system development; cell adhesion; homophilic cell adhesion