Rabbit anti-Human, Mouse Pbx1 Polyclonal Antibody | anti-PBX1 antibody

Pbx1

Cat. Num. AAA614906

• Gene Names: PBX1; MGC126627; DKFZp686B09108
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Purified by protein A and peptide affinity chromatography.
• Synonyms: Pbx1; Polyclonal Antibody; Anti -Pbx1; anti-PBX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total Pbx1a and Pbx1b proteins.
• Purity/Purification: Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-PBX1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:100
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to amino acids near the amino terminus of human Pbx1.
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from E10.5 mouse Pbx1 heterozygote or null emrbryos. Image kindly provided by Dr. Sheldon Rowan, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School.)

Western Blot (WB)

(Western blot analysis of extracts from A431, HeLa and HEL cells, using MBS614906.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells using MBS614906 (green). Actin filaments have been labeled with DY-554 phalloidin (red).)

Related Product Information for anti-PBX1 antibody

The Pbxs (Pre-B cell leukemia transcription factors) belong to the Three Amino Acid Loop Extension (TALE) family of homeodomain containing protein (1). Pbx1 has two isoforms, Pbx1a and 1b, that are divergent in sequence at the carboxy terminus, which is a result of alternative mRNA splicing. Pbx genes are ubiquitously expressed in both fetal and adult tissue, unlike other homeoselector genes that have restricted expression in development (1). Human pre-B cell acute leukemias are frequently associated with a t(1;19)(q23;p13.3) translocation that results in a fusion between the E2A and Pbx1 genes (2,3). Pbx1 genes are not normally expressed in lymphoid tissues, and the E2A-Pbx1 fusion protein is a potent transcription factor that drives aberrant gene expression.

Product Categories/Family for anti-PBX1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4505623
• NCBI GeneID: 5087
• NCBI Accession #: NP_002576.1
• NCBI GenBank Nucleotide #: NM_002585.3
• UniProt Accession #: P40424; Q5T488; Q68DD6
• Molecular Weight: 46,626 Da
• NCBI Official Full Name: pre-B-cell leukemia transcription factor 1 isoform 1
• NCBI Official Synonym Full Names: pre-B-cell leukemia homeobox 1
• NCBI Official Symbol: PBX1
• NCBI Official Synonym Symbols: MGC126627; DKFZp686B09108
• NCBI Protein Information: pre-B-cell leukemia transcription factor 1; OTTHUMP00000032205; OTTHUMP00000032206; homeobox protein PRL; homeobox protein PBX1
• UniProt Protein Name: Pre-B-cell leukemia transcription factor 1
• Protein Family: Pre-B-cell leukemia transcription factor
• UniProt Gene Name: PBX1
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PBX1_HUMAN

NCBI Description

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

PBX1: Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Forms a heterodimer with MEIS1 which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterotrimers with MEIS1 and a number of HOX proteins including HOXA9, HOXD4, HOXD9 and HOXD10. Interacts with PBXIP1. Expressed in all tissues except in cells of the B and T lineage. Belongs to the TALE/PBX homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity

Biological Process: spleen development; thymus development; transcription, DNA-dependent; somatic stem cell maintenance; embryonic hemopoiesis; adrenal gland development; embryonic skeletal development; negative regulation of transcription factor activity; anterior/posterior pattern formation; negative regulation of neuron differentiation; ureteric bud branching; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of ossification; sex differentiation; steroid biosynthetic process; proximal/distal pattern formation; embryonic limb morphogenesis