PBK Polyclonal Antibody | anti-PBK antibody

Anti-PBK Antibody

Cat. Num. AAA177884

• Gene Names: PBK; SPK; CT84; TOPK; HEL164; Nori-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: PBK; Polyclonal Antibody; Anti-PBK Antibody; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; CT84; Epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine activated killer T cell originated protein kinase; MAPKK like protein kinase; MAPKK-like protein kinase; Nori 3; Nori-3; Nori3; PDZ binding kinase; PDZ-binding kinase; Serine/threonine protein kinase; Spermatogenesis related protein kinase; Spermatogenesis-related protein kinase; SPK; T LAK cell originated protein kinase; T-LAK cell-originated protein kinase; TOPK; TOPK_HUMAN; anti-PBK antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 333

• Applicable Applications for anti-PBK antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human PBK recombinant protein (Position: N71-V322). Human PBK shares 89% amino acid (aa) sequence identity with mouse PBK.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- PBK Picoband antibody, MBS177884, Western blottingAll lanes: Anti PBK (MBS177884) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 36KDObserved bind size: 36KD )

Immunohistochemistry (IHC)

(Anti- PBK Picoband antibody, MBS177884, IHC(P)IHC(P): Mouse Testis Tissue )

Immunohistochemistry (IHC)

(Anti- PBK Picoband antibody, MBS177884, IHC(P)IHC(P): Rat Testis Tissue )

Immunohistochemistry (IHC)

(Anti- PBK Picoband antibody, MBS177884, IHC(P)IHC(P): Human Mammary Tissue )

Related Product Information for anti-PBK antibody

Description: Rabbit IgG polyclonal antibody for Lymphokine-activated killer T-cell-originated protein kinase(PBK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Lymphokine-activated killer T-cell-originated protein kinase, also known as TOPK or PBK, is an enzyme that in humans is encoded by the PBK gene. The protein encoded by this gene is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. It is mapped to 8p21.1. PBK can mediate cell growth through histone H3 modification. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis.

References

1. Abe Y, Matsumoto S, Kito K, Ueda N (Aug 2000). "Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells". J Biol Chem 275 (28): 21525-31. 2. "Entrez Gene: PBK PDZ binding kinase" 3. Park, J.-H., Lin, M.-L., Nishidate, T., Nakamura, Y., Katagiri, T. PDZ-binding kinase/T-LAK cell-originated protein kinase, a putative cancer/testis antigen with an oncogenic activity in breast cancer. Cancer Res. 66: 9186-9195, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 525507092
• NCBI GeneID: 55872
• NCBI Accession #: NP_001265874.1
• NCBI GenBank Nucleotide #: NM_001278945.1
• UniProt Accession #: Q96KB5; Q9NPD9; Q9NYL7; Q9NZK6; B4DX68; D3DST2
• Molecular Weight: 37,211 Da
• NCBI Official Full Name: lymphokine-activated killer T-cell-originated protein kinase isoform 2
• NCBI Official Synonym Full Names: PDZ binding kinase
• NCBI Official Symbol: PBK
• NCBI Official Synonym Symbols: SPK; CT84; TOPK; HEL164; Nori-3
• NCBI Protein Information: lymphokine-activated killer T-cell-originated protein kinase
• UniProt Protein Name: Lymphokine-activated killer T-cell-originated protein kinase
• Protein Family: Lymphokine-activated killer T-cell-originated protein kinase
• UniProt Gene Name: PBK
• UniProt Synonym Gene Names: TOPK; CT84; SPK
• UniProt Entry Name: TOPK_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PBK: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Interacts with DLG1 and TP53. Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Activated by phosphorylation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Protein type: Protein kinase, Other; Cancer Testis Antigen (CTA); EC 2.7.12.2; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; TOPK family

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nucleus

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity

Biological Process: mitosis; negative regulation of inflammatory response; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of stress-activated MAPK cascade; protein amino acid phosphorylation