Rabbit anti-Human PBK Polyclonal Antibody | anti-PBK antibody

PBK, CT (PDZ Binding Kinase, Cancer/testis Antigen 84, CT84, Lymphokine-activated Killer T-cell-originated Protein Kinase, MAPKK-like Protein Kinase, Nori-3, Spermatogenesis-related Protein Kinase, SPK, T-LAK Cell-originated Protein Kinase, TOPK) (Azide f

Cat. Num. AAA6492329

• Gene Names: PBK; SPK; CT84; TOPK; HEL164; Nori-3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PBK; Polyclonal Antibody; CT (PDZ Binding Kinase; Cancer/testis Antigen 84; CT84; Lymphokine-activated Killer T-cell-originated Protein Kinase; MAPKK-like Protein Kinase; Nori-3; Spermatogenesis-related Protein Kinase; SPK; T-LAK Cell-originated Protein Kinase; TOPK) (Azide f; EC=2.7.12.2; anti-PBK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PBK.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PBK antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PBK (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PBK (arrow) using rabbit polyclonal PBK Antibody (C-term C300).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK Antibody (C-term C300), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(PBK/TOPK Antibody (Mouse C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PBK/TOPK antibody detected the PBK/TOPK protein (arrow).)

Product Categories/Family for anti-PBK antibody

Antibodies; Protein Kinases

References

1. Nandi, A., et al., Blood Cells Mol. Dis. 32(1):240-245 (2004). 2. Simons-Evelyn, M., et al., Blood Cells Mol. Dis. 27(5):825-829 (2001). 3. Zhao, S., et al., Int. J. Biochem. Cell Biol. 33(6):631-636 (2001). 4. Abe, Y., et al., J. Biol. Chem. 275(28):21525-21531 (2000). 5. Gaudet, S., et al., Proc. Natl. Acad. Sci. USA 97(10):5167-5172 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 18490991
• NCBI GeneID: 55872
• NCBI Accession #: NP_060962
• NCBI GenBank Nucleotide #: NM_018492.3
• UniProt Accession #: Q96KB5; Q9NPD9; Q9NYL7; Q9NZK6; B4DX68; D3DST2
• Molecular Weight: 38.6 kDa (346aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• NCBI Official Full Name: lymphokine-activated killer T-cell-originated protein kinase isoform 1
• NCBI Official Synonym Full Names: PDZ binding kinase
• NCBI Official Symbol: PBK
• NCBI Official Synonym Symbols: SPK; CT84; TOPK; HEL164; Nori-3
• NCBI Protein Information: lymphokine-activated killer T-cell-originated protein kinase
• UniProt Protein Name: Lymphokine-activated killer T-cell-originated protein kinase
• Protein Family: Lymphokine-activated killer T-cell-originated protein kinase
• UniProt Gene Name: PBK
• UniProt Synonym Gene Names: TOPK; CT84; SPK
• UniProt Entry Name: TOPK_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PBK: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Interacts with DLG1 and TP53. Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Activated by phosphorylation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Protein type: Protein kinase, Other; Cancer Testis Antigen (CTA); Protein kinase, Ser/Thr (non-receptor); EC 2.7.12.2; Kinase, protein; Other group; TOPK family

Chromosomal Location of Human Ortholog: 8p21.2

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: mitosis; negative regulation of protein amino acid phosphorylation; negative regulation of inflammatory response; negative regulation of stress-activated MAPK cascade; protein amino acid phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process