Western Blot (WB)
(Western blot analysis of PBK (arrow) using rabbit polyclonal PBK Antibody (C-term C300) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human PBK Polyclonal Antibody
PBK, CT (PDZ Binding Kinase, Cancer/testis Antigen 84, CT84, Lymphokine-activated Killer T-cell-originated Protein Kinase, MAPKK-like Protein Kinase, Nori-3, Spermatogenesis-related Protein Kinase, SPK, T-LAK Cell-originated Protein Kinase, TOPK)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
PBK; Polyclonal Antibody; CT (PDZ Binding Kinase; Cancer/testis Antigen 84; CT84; Lymphokine-activated Killer T-cell-originated Protein Kinase; MAPKK-like Protein Kinase; Nori-3; Spermatogenesis-related Protein Kinase; SPK; T-LAK Cell-originated Protein Kinase; TOPK); Anti -PBK; anti-PBK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PBK.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-PBK antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Immunogen
Synthetic peptide selected from the C-terminal region of human PBK (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PBK (arrow) using rabbit polyclonal PBK Antibody (C-term C300) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of PBK (arrow) using rabbit polyclonal PBK Antibody (C-term C300) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK Antibody (C-term C300) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK Antibody (C-term C300) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(PBK/TOPK Antibody (Mouse C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PBK/TOPK antibody detected the PBK/TOPK protein (arrow).)
Western Blot (WB)
(PBK/TOPK Antibody (Mouse C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PBK/TOPK antibody detected the PBK/TOPK protein (arrow).)
Related Product Information for anti-PBK antibody
PBK is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
Product Categories/Family for anti-PBK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
PBK
Protein Family
Similar Products
Product Notes
The PBK (Catalog #AAA630866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PBK, CT (PDZ Binding Kinase, Cancer/testis Antigen 84, CT84, Lymphokine-activated Killer T-cell-originated Protein Kinase, MAPKK-like Protein Kinase, Nori-3, Spermatogenesis-related Protein Kinase, SPK, T-LAK Cell-originated Protein Kinase, TOPK) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PBK can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the PBK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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