Testing Data
Rabbit Paxillin (Ser-178) Polyclonal Antibody | anti-Pxn antibody
Anti-Paxillin (Ser-178), phospho-specific
Gene Names
Pxn; Pxn
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot
Purity
Affinity Purified
Synonyms
Paxillin (Ser-178); Polyclonal Antibody; Anti-Paxillin (Ser-178); phospho-specific; Paxillin; anti-Pxn antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody detects a 68kDa* protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Sequence Length
586
Applicable Applications for anti-Pxn antibody
ELISA, Western Blot
Application Notes
ELISA: 1:2000
Western Blot: 1:500
End user should determine an optimum working dilution for their particular applciations and experiments.
Western blot membranes were incubated with diluted anitbody in 5% non-fat milk, PBS, 0.04% Tween200 for20 for 1 hour at room temperature.
Western Blot: 1:500
End user should determine an optimum working dilution for their particular applciations and experiments.
Western blot membranes were incubated with diluted anitbody in 5% non-fat milk, PBS, 0.04% Tween200 for20 for 1 hour at room temperature.
Immunogen
Phospho-Paxillin (Ser-178) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
Preparation and Storage
Store at -20°C. Do not aliquot. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for anti-Pxn antibody
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.
References
• Huang, C. et al. (2003) Nature 424:219-223.
• Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
• Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.
• Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
• Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63888 Da
NCBI Official Full Name
paxillin
NCBI Official Synonym Full Names
paxillin
NCBI Official Symbol
Pxn
NCBI Official Synonym Symbols
Pxn
NCBI Protein Information
paxillin; myocardial ischemic preconditioning associated protein 7
UniProt Protein Name
Paxillin
Protein Family
UniProt Gene Name
Pxn
UniProt Entry Name
PAXI_RAT
Uniprot Description
Function: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion)
Subunit structure: Binds to vinculin, to the SH3 domain of c-SRC and to focal adhesion kinase. Interacts with GIT1 and NUDT16L1/SDOS. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2 and PDCD10
By similarity. Interacts with PARVA and TGFB1I1. Ref.2 Ref.3
Subcellular location: Cytoplasm › cytoskeleton
By similarity. Cell junction › focal adhesion
Post-translational modification: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens
By similarity. Ref.4
Sequence similarities: Belongs to the paxillin family.Contains 4 LIM zinc-binding domains.
Research Articles on Pxn
Similar Products
Product Notes
The Pxn pxn (Catalog #AAA472047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Paxillin (Ser-178), phospho-specific reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Paxillin (Ser-178) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. ELISA: 1:2000 Western Blot: 1:500 End user should determine an optimum working dilution for their particular applciations and experiments. Western blot membranes were incubated with diluted anitbody in 5% non-fat milk, PBS, 0.04% Tween200 for20 for 1 hour at room temperature. Researchers should empirically determine the suitability of the Pxn pxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paxillin (Ser-178), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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