Rabbit anti-Human Paxillin Polyclonal Antibody | anti-Pxn antibody

Anti-Paxillin Antibody

Cat. Num. AAA417093

• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity purified
• Synonyms: Paxillin; Polyclonal Antibody; Anti-Paxillin Antibody; Anti-Paxillin (Ab-88) Antibody; anti-Pxn antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Paxillin (Ab-88) Antibody detects endogenous levels of total Paxillin protein.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 586

• Applicable Applications for anti-Pxn antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB:1:500~1:1000; IF:1:100~1:200
• Storage Buffer: Rabbit IgG in phosphate buffered saline (without Mg< sup>2< /sup>+ and Ca< sup>2< /sup>+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Immunogen: The antibody was produced against synthesized non-phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 88 (P-V-YP-G-S).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 58865866
• NCBI GeneID: 360820
• NCBI Accession #: NP_001012147.1
• NCBI GenBank Nucleotide #: NM_001012147.1
• Molecular Weight: 64,019 Da
• NCBI Official Full Name: paxillin
• NCBI Official Synonym Full Names: paxillin
• NCBI Official Symbol: Pxn
• NCBI Protein Information: paxillin; myocardial ischemic preconditioning associated protein 7
• UniProt Protein Name: Paxillin
• Protein Family: Paxillin
• UniProt Gene Name: Pxn
• UniProt Entry Name: PAXI_RAT

Uniprot Description

PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.

Protein type: Cytoskeletal; Adaptor/scaffold; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; cytoskeleton; focal adhesion; lamellipodium; cytoplasm; leading edge; stress fiber; plasma membrane; cell cortex; nucleus

Molecular Function: integrin binding; zinc ion binding; beta-catenin binding; protein complex binding; BH4 domain binding; protein kinase binding; vinculin binding

Biological Process: lamellipodium biogenesis; focal adhesion formation; integrin-mediated signaling pathway; regulation of cell shape; peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; activation of MAPK activity; cell-matrix adhesion; positive regulation of protein kinase activity; transforming growth factor beta receptor signaling pathway; cytoskeleton organization and biogenesis