Mouse anti-Human LOX / Lysyl Oxidase Monoclonal Antibody | anti-LOX antibody

Anti-LOX / Lysyl Oxidase Antibody (clone 9A11) IHC-plus

Cat. Num. AAA249296

• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G purified
• Synonyms: LOX / Lysyl Oxidase; Monoclonal Antibody; Anti-LOX / Lysyl Oxidase Antibody (clone 9A11) IHC-plus; Mouse Monoclonal [clone 9A11] (IgG1) to Human LOX / Lysyl Oxidase; Human LOX / Lysyl Oxidase; Lysyl oxidase; Protein-lysine 6-oxidase; anti-LOX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9A11
• Specificity: Human Lysyl Oxidase / LOX
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.8 mg/ml (varies by lot)
• Sequence Length: 417

• Applicable Applications for anti-LOX antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:100); IF (1:50 - 1:100); IHC-P (10 ug/ml); WB (1:2000)
• Immunogen: LOX / Lysyl Oxidase antibody was raised against human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell.
• Immunogen Description: Human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Lysyl Oxidase / LOX antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescent staining of HepG2 cells using anti-LOX mouse monoclonal antibody.)

Immunofluorescence (IF)

(Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.)

Flow Cytometry (FC/FACS)

(HEK293T cells transfected with either pCMV6-ENTRY LOX (Red) or empty vector control plasmid (Blue) were immunostained with anti-LOX mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.)

Flow Cytometry (FC/FACS)

(Flow cytometry of Jurkat cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-LOX antibody, (Red) compared to a nonspecific negative control antibody (Blue).)

Related Product Information for anti-LOX antibody

LOX Antibody, Lysyl oxidase Antibody, Protein-lysine 6-oxidase Antibody Description: Lysyl Oxidase / LOX is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I).

NCBI and Uniprot Product Information

• NCBI GI #: 20149540
• NCBI GeneID: 4015
• NCBI Accession #: NP_002308.2
• NCBI GenBank Nucleotide #: NM_002317.6
• UniProt Accession #: P28300; Q5FWF0; B2R5Q3
• Molecular Weight: 46,944 Da
• NCBI Official Full Name: protein-lysine 6-oxidase isoform 1 preproprotein
• NCBI Official Synonym Full Names: lysyl oxidase
• NCBI Official Symbol: LOX
• NCBI Protein Information: protein-lysine 6-oxidase
• UniProt Protein Name: Protein-lysine 6-oxidase
• Protein Family: Linoleate 9/13-lipoxygenase
• UniProt Gene Name: LOX
• UniProt Entry Name: LYOX_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression. [provided by RefSeq, Nov 2015]

Uniprot Description

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Secreted, signal peptide; Secreted; EC 1.4.3.13; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: collagen; extracellular region; extracellular space; nucleus; proteinaceous extracellular matrix

Molecular Function: copper ion binding; protein binding; protein-lysine 6-oxidase activity

Biological Process: collagen fibril organization; elastic fiber assembly; extracellular matrix organization and biogenesis; heart development; lung development; protein modification process; response to drug; response to steroid hormone stimulus; wound healing

Disease: Cutis Laxa, Autosomal Recessive, Type Ia